Fig. 6
From: Nitric oxide contributes to methane-induced osmotic stress tolerance in mung bean
In vivo analysis revealed that CH4-mediated S-nitrosylation was sensitive to PTIO. Mung bean seeds were presoaked with solutions containing 1.3 mM CH4, 100 μM SNP, 500 μM PTIO, alone or their combinations for 12 h, and then shifted to 20% PEG-6000 for another 48 h. Biotin labeled proteins were detected by Western blot with anti-biotin antibodies (a). Meanwhile, the equal protein amounts were evaluated by Coomassie staining (b) after SDS-PAGE separation. Numbers on the left of the panels indicate the position of the protein markers in kDa. The gels are representative of at lest five replicates per experiment