Fig. 7From: A Lotus japonicus E3 ligase interacts with the Nod Factor Receptor 5 and positively regulates nodulationLjPUB13 specifically ubiquitinates the Nod Factor receptor NFR5. LjPUB13 was purified as GST fusion, while the cytoplasmic regions of NFR1 and NFR5 were purified as HIS fusion proteins. a, b The NFR5 ubiquitination was detected by an anti-HIS antibody, using two different E2 ligases: UbcH6 (a) and UbcH5b (b). No ubiquitination was observed when NFR1 was used as a substrate. c The presence of LjPUB13 at the ubiquitination reactions was detected by an anti-GST antibody (input). d Purified NFR1-HIS and NFR5-HIS used at the ubiquitination reactions. The experiments were repeated three times with similar resultsBack to article page