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Fig. 7 | BMC Plant Biology

Fig. 7

From: A Lotus japonicus E3 ligase interacts with the Nod Factor Receptor 5 and positively regulates nodulation

Fig. 7

LjPUB13 specifically ubiquitinates the Nod Factor receptor NFR5. LjPUB13 was purified as GST fusion, while the cytoplasmic regions of NFR1 and NFR5 were purified as HIS fusion proteins. a, b The NFR5 ubiquitination was detected by an anti-HIS antibody, using two different E2 ligases: UbcH6 (a) and UbcH5b (b). No ubiquitination was observed when NFR1 was used as a substrate. c The presence of LjPUB13 at the ubiquitination reactions was detected by an anti-GST antibody (input). d Purified NFR1-HIS and NFR5-HIS used at the ubiquitination reactions. The experiments were repeated three times with similar results

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