Fig. 5
LjPUB13 interacts with LjBAK1, LjFLS2 and NFR5 in vitro. a In vitro binding assay of the HIS-tagged LjPUB13 with the GST-fused cytoplasmic regions of LjBAK1 and LjFLS2. LjPUB13 interacts with LjBAK1cyt and LjFLS2cyt. b In vitro binding assays of the GST-fused LjPUB13 and LjBAK1cyt with the HIS-tagged cytoplasmic regions of the Nod Factor receptors NFR1 and NFR5. LjPUB13 interacts strongly with NFR5cyt, while the LjPUB13-NFR1cyt interaction is undetectable. Sometimes a weak interaction of LjPUB13-NFR1cyt was observed but this result was not always reproducible. LjBAK1cyt also interacts with NFR5cyt, but no interaction was ever observed between LjBAK1cyt and NFR1cyt. After GST pulldown, bead-bound proteins were analyzed by immunoblotting using anti-HIS and anti-GST antibodies. These experiments were repeated five times