Fig. 1

LjPUB13 is an active E3 ubiquitin ligase. a L. japonicus PUB13 gene structure. PUB13 protein contains a U-box motif and an ARM-repeat domain. The pub13 mutants carry LORE1 insertions in the coding region of the PUB13 gene. The positions of the LORE1 insertions in the pub13 mutants are marked by arrows. Numbers indicate nucleotides. b LjPUB13 auto-ubiquitination. PUB13 was purified as a GST fusion protein. The ubiquitination was detected by both anti-GST and anti-Ub antibodies. UbcH6 was used as the E2 enzyme in the reactions. The experiment was repeated four times with similar results