The rare orange-red colored Euphorbia pulcherrima cultivar ‘Harvest Orange’ shows a nonsense mutation in a flavonoid 3’-hydroxylase allele expressed in the bracts

Table 1 The anthocyanins in bracts of poinsettia cultivars and their respective concentration as determined by HPLC and LC-MS analysis of extracts

Anthocyanin composition [mg/g FW]	‘Christmas Beauty’	‘Christmas Feeling’	‘Harvest Orange’	‘Premium Red’
Total anthocyanins	29.7 ± 1.7	29.0 ± 0.5	15.4 ± 0.7	9.3 ± 3.9
Pelargonidin 3-O-glucoside	6.4 ± 0.4	5.2 ± 0.9	9.9 ± 0.1	3.8 ± 0.6
Pelargonidin 3-O-rutinoside	3.0 ± 0.2	2.9 ± 0.6	5.0 ± 0.6	2.0 ± 0.1
Other pelargonidin derivatives^a	≥ 0.02	≥0.01	≥0.02	≥0.02
Total pelargonidin based anthocyanins	9.4 ± 0.6	8.2 ± 0.1	14.9 ± 0.2	7.9 ± 3.6
Cyanidin-3-O-glucoside	5.3 ± 0.2	5.0 ± 0.9	0.06 ± 0.1	0.4 ± 0.1
Cyanidin-3-O-rutinoside	9.9 ± 0.4	10.4 ± 0.2	n.d.	0.7 ± 0.1
Cyanidin-3-O-galactoside	5.0 ± 0.7	5.4 ± 0.7	n.d.	0.2 ± 0.03
Other cyanidin derivatives^b	≥0.06	≥ 0.05	n.d.	≥0.01
Total cyanidin based anthocyanins	20.3 ± 1.4	20.9 ± 0.3	0.06 ± 0.1	1.3 ± 0.2
Total delphinidin based anthocyanins	≥0.01	≥0.01	n.d.	≥0.01

^aPelargonidin 3-O-(6”malonylglucoside), Pelargonidin 3-O-(6”malonyldihexosid) ^bCyanidin-3-O-xyloside

ISSN: 1471-2229