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Fig. 5 | BMC Plant Biology

Fig. 5

From: The rare orange-red colored Euphorbia pulcherrima cultivar ‘Harvest Orange’ shows a nonsense mutation in a flavonoid 3’-hydroxylase allele expressed in the bracts

Fig. 5

Amplification of F3′H with the primer pair EpF3′H_fraF and EpF3′H_fraR (Additional file 5: Table S4) flanking the variable region at the N-terminal end using genomic DNA (a) and cDNA (b) from the four poinsettia cultivars ‘Harvest Orange’ (HO), ‘Premium Red’ (PR), Christmas (CB) and ‘Christmas Feeling’ (CF). For cv. ‘Harvest Orange’, amplification from gDNA delivered two fragments of the expected size (calculated values: 109 and 138 bp), whereas only the larger fragment was obtained with cDNA. With gDNA and cDNA from the other cultivars only a single fragment of the smaller size was obtained. Size marker (M) was the 2-Log DNA Ladder (New England Biolabs, UK) with digested DNA fragments ranging from 100 bp to 10 kbp (100 bp steps between 100 and 1000); 100 and 200 bp fragments are highlighted on the gel with red arrows

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BMC Plant Biology

ISSN: 1471-2229

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