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Table 3 DAP spots and their identification by MALDI-TOF/TOF-MS in leaves from C. grandis seedlings submitted to pH 2.5, pH 3 or pH 6 for 9 months

From: Low pH-responsive proteins revealed by a 2-DE based MS approach and related physiological responses in Citrus leaves

Spot No.a

Protein identity

Accession No.

Mr (kDa)/pI theor.

Mr (kDa)/pI exp.

Protein score

Peptide ions

NMPb

Ratioc

Coveredd sequence (%)

Charge

pH 2.5

pH 3

pH 6

Antioxidation and detoxification

 G2

Copper/zinc superoxide dismutase 2, chloroplastic

Cs8g15520.1

26.00/6.52

22.72/6.05

341

119

8

2.51 ± 0.06a

2.42 ± 0.24a

1.00 ± 0.17b

7

1

 G11

Aldehyde dehydrogenase family 2 member B4, mitochondrial

Cs5g05240.1

58.91/7.11

75.4/5.31

409

115

27

2.48 ± 0.20a

0.33 ± 0.06c

1.00 ± 0.08b

25

1

 G40

Apolipoprotein D

Cs4g01600.1

21.52/6.33

32.64/6.84

264

106

11

1.57 ± 0.22a

0.62 ± 0.04b

1.00 ± 0.10b

10

1

 G15

Phosphomannomutase 1

orange1.1 t00331.1

28.16/6.19

59.09/5.85

561

122

25

0.48 ± 0.05b

1.01 ± 0.02a

1.00 ± 0.05a

23

1

 G1

NADP-dependent alkenal double bond reductase P2

Cs5g21010.1

36.04/6.16

82.02/5.3

435

116

18

0.18 ± 0.04b

0.38 ± 0.07b

1.00 ± 0.29a

16

1

 G3

Thioredoxin-like protein CDSP32, chloroplastic

Cs3g26690.1

39.02/8.59

49.64/6.62

472

106

18

0.36 ± 0.05c

0.66 ± 0.09b

1.00 ± 0.08a

16

1

 G4

Thioredoxin-like protein CDSP32, chloroplastic

Cs3g26690.1

39.02/8.59

49.46/6.85

477

106

20

0.22 ± 0.01b

0.83 ± 0.15a

1.00 ± 0.22a

18

1

 G8

Ferredoxin-thioredoxin reductase catalytic chain, chloroplastic

Cs6g20130.1

16.57/6.29

24.68/5.07

130

61

7

0.25 ± 0.05b

0.42 ± 0.10b

1.00 ± 0.08a

6

1

 G23

Protein disulfide isomerase-like 1–1

Cs3g19790.1

55.62/4.84

83.51/4.91

625

95

31

0.43 ± 0.06b

0.80 ± 0.09ab

1.00 ± 0.16a

28

1

 G39

Annexin A13

Cs3g18360.1

36.10/5.55

28.12/6.6

422

82

23

0.18 ± 0.05c

0.52 ± 0.04b

1.00 ± 0.07a

21

1

 G36

Nucleoside diphosphate kinase II, chloroplastic

Cs5g06840.1

25.53/9.35

25.98/6.32

467

106

13

0.07 ± 0.02b

0.25 ± 0.02b

1.00 ± 0.15a

12

1

 G5

Betaine aldehyde dehydrogenase 1, chloroplastic

Cs5g04880.1

54.95/5.40

24.08/4.24

236

116

12

0.10 ± 0.05c

0.52 ± 0.10b

1.00 ± 0.01a

11

1

Carbohydrate and energy metabolism

 G7

RuBisCO large subunit-binding protein subunit alpha, chloroplastic; Chaperonin 60 subunit alpha 1

Cs8g16040.1

61.50/5.23

46.22/5.1

215

77

23

2.75 ± 0.28a

1.58 ± 0.11b

1.00 ± 0.06b

21

1

 G10

RuBisCO large subunit-binding protein subunit alpha, chloroplastic; Chaperonin 60 subunit α 1

Cs8g16040.1

61.50/5.23

82.35/5.02

783

126

37

0.35 ± 0.09b

0.99 ± 0.13a

1.00 ± 0.09a

34

1

 G12e

Ribulose bisphosphate carboxylase/oxygenase activase 1, chloroplastic

Cs7g31800.2

41.38/5.07

64.04/5.4

219

97

10

0.14 ± 0.02b

0.31 ± 0.05b

1.00 ± 0.08a

9

1

 G19

Ferredoxin--NADP reductase, leaf isozyme, chloroplastic

Cs1g25510.1

40.48/8.68

54.2/6.92

289

70

23

0.44 ± 0.05b

0.53 ± 0.06b

1.00 ± 0.12a

21

1

 G13

Rhodanese-like domain-containing protein 4A, chloroplastic

orange1.1 t00475.2

46.75/5.59

69.08/5.26

507

130

24

0.41 ± 0.06b

1.00 ± 0.12a

1.00 ± 0.18a

22

1

 G16

2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, chloroplastic

Cs3g01420.1

32.59/8.29

46.72/5.75

321

95

12

0.34 ± 0.06b

0.88 ± 0.13a

1.00 ± 0.15a

11

1

 G17

Probable 6-phosphogluconolactonase 2

orange1.1 t02542.1

35.38/6.24

81.27/5.72

454

133

15

0.34 ± 0.04b

2.61 ± 0.45a

1.00 ± 0.01b

14

1

 G18

Dihydroxyacetone kinase 2

orange1.1 t02644.1

61.75/5.74

81.59/6.13

342

95

19

2.30 ± 0.56a

1.66 ± 0.14ab

1.00 ± 0.04b

17

1

 G9

Probable fructokinase-1

Cs5g22920.1

35.11/4.98

59.41/5.02

718

137

22

0.49 ± 0.10b

0.58 ± 0.05b

1.00 ± 0.07a

20

1

 G33

DNA-damage-repair/toleration protein DRT102; ribose-5-phosphate isomerase B

Cs3g11320.1

33.52/5.25

52.44/5.28

279

104

15

0.27 ± 0.06b

0.79 ± 0.05a

1.00 ± 0.20a

14

1

Protein and amino acid metabolism

 G26

Peptidyl-prolyl cis-trans isomerase FKBP62

Cs7g16620.3

63.83/5.19

87.75/5.46

344

81

21

0.23 ± 0.02c

0.79 ± 0.07b

1.00 ± 0.03a

19

1

 G21

Peptidyl-prolyl cis-trans isomerase CYP38, chloroplastic

Cs2g28260.1

48.02/5.00

64.55/4.61

231

80

18

0.20 ± 0.06c

0.47 ± 0.04b

1.00 ± 0.05a

16

1

 G30

Ankyrin repeat domain-containing protein 2

orange1.1 t02270.1

64.18/5.29

64.12/4.57

261

110

16

0.16 ± 0.04b

0.72 ± 0.09a

1.00 ± 0.11a

15

1

 G31

Ankyrin repeat domain-containing protein 2

orange1.1 t02270.1

64.18/5.29

65.14/4.51

228

120

13

0.30 ± 0.13b

1.13 ± 0.16a

1.00 ± 0.21a

12

1

 G20

SKP1-like protein 10

Cs3g26510.1

17.82/4.56

33.74/4.58

105

60

11

0.16 ± 0.02b

0.27 ± 0.04b

1.00 ± 0.13a

10

1

 G29

Subtilisin-like protease SDD1

Cs1g17350.1

82.90/5.86

49.48/6.95

311

107

12

0.25 ± 0.06b

0.07 ± 0.01b

1.00 ± 0.09a

11

1

 G24

26S protease regulatory subunit 6B homolog

Cs1g08770.1

46.49/5.42

72.36/5.68

425

87

27

1.24 ± 0.12a

0.42 ± 0.05b

1.00 ± 0.12a

25

1

 G6

Proline iminopeptidase

Cs8g03250.1

44.67/5.70

60.15/5.58

463

116

23

3.44 ± 0.42a

2.21 ± 0.41b

1.00 ± 0.05c

21

1

 G25

Glutamine synthetase cytosolic isozyme

Cs9g05680.1

47.86/6.29

65.88/5.51

181

82

16

0.07 ± 0.02c

0.61 ± 0.12b

1.00 ± 0.14a

15

1

 G34

Dihydropyrimidine dehydrogenase [NADP(+)]

orange1.1 t02649.1

46.01/6.29

66.1/5.75

189

96

16

0.28 ± 0.04b

0.50 ± 0.02b

1 ± 0.17a

15

1

 G27

Argininosuccinate synthase, chloroplastic

Cs5g07120.1

54.38/6.50

70.63/5.94

371

112

26

2.36 ± 0.13a

0.90 ± 0.14b

1.00 ± 0.18b

24

1

 G28

3-isopropylmalate dehydratase large subunit 1

Cs5g35290.1

55.66/6.68

76.63/6.29

124

72

15

3.06 ± 0.05a

1.55 ± 0.12b

1.00 ± 0.08c

14

1

 G22

Unknown protein DS12 from 2D-PAGE of leaf, chloroplastic; ACT domain-containing protein, putative, expressed

orange1.1 t04488.3

26.98/4.94

51.32/4.76

318

89

16

0.41 ± 0.11b

1.08 ± 0.26a

1.00 ± 0.02a

15

1

Signal transduction

 G32

Plasma membrane-associated cation-binding protein 1

Cs2g12010.1

23.34/5.03

51.44/5.25

113

37

12

0.19 ± 0.04b

0.39 ± 0.04b

1.00 ± 0.09a

11

1

Nucleic acid metabolism

 G35

Adenylosuccinate synthetase 1, chloroplastic

orange1.1 t03395.1

53.23/6.40

70.64/6.01

568

101

25

0.25 ± 0.01b

0.42 ± 0.09b

1 ± 0.22a

23

1

Lipid metabolism

 G37

Lipase/lipooxygenase, PLAT/LH2 family protein

Cs1g01370.1

19.62/4.80

22.28/4.22

213

134

7

0.15 ± 0.03c

0.46 ± 0.07b

1 ± 0.03a

6

1

 G38

Lipase/lipooxygenase, PLAT/LH2 family protein

Cs1g01370.1

19.62/4.80

44.79/6.6

222

137

7

0.33 ± 0.07b

0.48 ± 0.06b

1 ± 0.003a

6

1

 G14

Allene oxide cyclase 1, chloroplastic

Cs6g18900.1

27.51/8.63

34.12/5.73

371

83

13

0.19 ± 0.06c

0.64 ± 0.03b

1.00 ± 0.11a

12

1

Cellular transport

 G41

V-type proton ATPase catalytic subunit A isoform 1

Cs1g10270.1

68.68/5.29

59.4/6.96

635

100

40

1.50 ± 0.16a

0.65 ± 0.07b

1.00 ± 0.08b

36

1

Others

 G42

Putative uncharacterized protein Sb01g045410

Cs4g06170.1

26.81/8.90

31.73/5.39

228

82

11

0.13 ± 0.04b

0.22 ± 0.03b

1.00 ± 0.14a

10

1

 G43

Putative uncharacterized protein Sb09g010000

Cs8g19010.1

55.68/7.66

80.33/6.05

285

118

20

0.46 ± 0.05c

1.18 ± 0.06a

1.00 ± 0.02b

18

1

 G44

Uncharacterized protein At2g37660, chloroplastic

Cs6g06590.1

36.01/9.43

45/6.92

485

136

14

0.19 ± 0.03c

0.58 ± 0.07b

1.00 ± 0.15a

13

1

Unidentified protein spots

 G45

Kinesin-like protein KIF21A

Cs3g17220.3

108.17/6.47

25.06/4.49

52

/

28

0.27 ± 0.07b

0.39 ± 0.07b

1.00 ± 0.25a

25

1

 G46

Actinidain

Cs3g23180.1

51.42/5.37

52.44/4.62

55

55

2

0.35 ± 0.01b

0.97 ± 0.25a

1.00 ± 0.13a

2

1

 G47

Peptide methionine sulfoxide reductase B2, chloroplastic

Cs8g03090.1

17.59/10.55

20.63/5.27

51

24

8

0.10 ± 0.009b

0.34 ± 0.14b

1.00 ± 0.13a

7

1

 G48

Putative uncharacterized protein

Cs7g03150.1

15.56/6.22

85.49/5.56

55

29

7

0.35 ± 0.06b

0.85 ± 0.05a

1.00 ± 0.13a

6

1

  1. aSpot number corresponds to the 2-DE imagines
  2. bNMP means the number of matched peptides in Fig. 5
  3. cRatio means the ratio of pH 2.5 and pH 3 to pH 6.0; Different letters within a row indicate significant differences at P < 0.05
  4. dCovered sequence (%) means the ratio of the number of amino of the matched peptides to the number of amino acids of the full-length protein
  5. eLow pH-responsive proteins shared by the two Citrus species were highlighted in bold
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BMC Plant Biology

ISSN: 1471-2229

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