Spot No.a | Protein identity | Accession No. | Mr (kDa)/pI theor. | Mr (kDa)/pI exp. | Protein score | Peptide ions | NMPb | Ratioc | Coveredd sequence (%) | Charge | ||
---|---|---|---|---|---|---|---|---|---|---|---|---|
pH 2.5 | pH 3 | pH 6 | ||||||||||
Antioxidation and detoxification | ||||||||||||
 G2 | Copper/zinc superoxide dismutase 2, chloroplastic | Cs8g15520.1 | 26.00/6.52 | 22.72/6.05 | 341 | 119 | 8 | 2.51 ± 0.06a | 2.42 ± 0.24a | 1.00 ± 0.17b | 7 | 1 |
 G11 | Aldehyde dehydrogenase family 2 member B4, mitochondrial | Cs5g05240.1 | 58.91/7.11 | 75.4/5.31 | 409 | 115 | 27 | 2.48 ± 0.20a | 0.33 ± 0.06c | 1.00 ± 0.08b | 25 | 1 |
 G40 | Apolipoprotein D | Cs4g01600.1 | 21.52/6.33 | 32.64/6.84 | 264 | 106 | 11 | 1.57 ± 0.22a | 0.62 ± 0.04b | 1.00 ± 0.10b | 10 | 1 |
 G15 | Phosphomannomutase 1 | orange1.1 t00331.1 | 28.16/6.19 | 59.09/5.85 | 561 | 122 | 25 | 0.48 ± 0.05b | 1.01 ± 0.02a | 1.00 ± 0.05a | 23 | 1 |
 G1 | NADP-dependent alkenal double bond reductase P2 | Cs5g21010.1 | 36.04/6.16 | 82.02/5.3 | 435 | 116 | 18 | 0.18 ± 0.04b | 0.38 ± 0.07b | 1.00 ± 0.29a | 16 | 1 |
 G3 | Thioredoxin-like protein CDSP32, chloroplastic | Cs3g26690.1 | 39.02/8.59 | 49.64/6.62 | 472 | 106 | 18 | 0.36 ± 0.05c | 0.66 ± 0.09b | 1.00 ± 0.08a | 16 | 1 |
 G4 | Thioredoxin-like protein CDSP32, chloroplastic | Cs3g26690.1 | 39.02/8.59 | 49.46/6.85 | 477 | 106 | 20 | 0.22 ± 0.01b | 0.83 ± 0.15a | 1.00 ± 0.22a | 18 | 1 |
 G8 | Ferredoxin-thioredoxin reductase catalytic chain, chloroplastic | Cs6g20130.1 | 16.57/6.29 | 24.68/5.07 | 130 | 61 | 7 | 0.25 ± 0.05b | 0.42 ± 0.10b | 1.00 ± 0.08a | 6 | 1 |
 G23 | Protein disulfide isomerase-like 1–1 | Cs3g19790.1 | 55.62/4.84 | 83.51/4.91 | 625 | 95 | 31 | 0.43 ± 0.06b | 0.80 ± 0.09ab | 1.00 ± 0.16a | 28 | 1 |
 G39 | Annexin A13 | Cs3g18360.1 | 36.10/5.55 | 28.12/6.6 | 422 | 82 | 23 | 0.18 ± 0.05c | 0.52 ± 0.04b | 1.00 ± 0.07a | 21 | 1 |
 G36 | Nucleoside diphosphate kinase II, chloroplastic | Cs5g06840.1 | 25.53/9.35 | 25.98/6.32 | 467 | 106 | 13 | 0.07 ± 0.02b | 0.25 ± 0.02b | 1.00 ± 0.15a | 12 | 1 |
 G5 | Betaine aldehyde dehydrogenase 1, chloroplastic | Cs5g04880.1 | 54.95/5.40 | 24.08/4.24 | 236 | 116 | 12 | 0.10 ± 0.05c | 0.52 ± 0.10b | 1.00 ± 0.01a | 11 | 1 |
Carbohydrate and energy metabolism | ||||||||||||
 G7 | RuBisCO large subunit-binding protein subunit alpha, chloroplastic; Chaperonin 60 subunit alpha 1 | Cs8g16040.1 | 61.50/5.23 | 46.22/5.1 | 215 | 77 | 23 | 2.75 ± 0.28a | 1.58 ± 0.11b | 1.00 ± 0.06b | 21 | 1 |
 G10 | RuBisCO large subunit-binding protein subunit alpha, chloroplastic; Chaperonin 60 subunit α 1 | Cs8g16040.1 | 61.50/5.23 | 82.35/5.02 | 783 | 126 | 37 | 0.35 ± 0.09b | 0.99 ± 0.13a | 1.00 ± 0.09a | 34 | 1 |
 G12e | Ribulose bisphosphate carboxylase/oxygenase activase 1, chloroplastic | Cs7g31800.2 | 41.38/5.07 | 64.04/5.4 | 219 | 97 | 10 | 0.14 ± 0.02b | 0.31 ± 0.05b | 1.00 ± 0.08a | 9 | 1 |
 G19 | Ferredoxin--NADP reductase, leaf isozyme, chloroplastic | Cs1g25510.1 | 40.48/8.68 | 54.2/6.92 | 289 | 70 | 23 | 0.44 ± 0.05b | 0.53 ± 0.06b | 1.00 ± 0.12a | 21 | 1 |
 G13 | Rhodanese-like domain-containing protein 4A, chloroplastic | orange1.1 t00475.2 | 46.75/5.59 | 69.08/5.26 | 507 | 130 | 24 | 0.41 ± 0.06b | 1.00 ± 0.12a | 1.00 ± 0.18a | 22 | 1 |
 G16 | 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, chloroplastic | Cs3g01420.1 | 32.59/8.29 | 46.72/5.75 | 321 | 95 | 12 | 0.34 ± 0.06b | 0.88 ± 0.13a | 1.00 ± 0.15a | 11 | 1 |
 G17 | Probable 6-phosphogluconolactonase 2 | orange1.1 t02542.1 | 35.38/6.24 | 81.27/5.72 | 454 | 133 | 15 | 0.34 ± 0.04b | 2.61 ± 0.45a | 1.00 ± 0.01b | 14 | 1 |
 G18 | Dihydroxyacetone kinase 2 | orange1.1 t02644.1 | 61.75/5.74 | 81.59/6.13 | 342 | 95 | 19 | 2.30 ± 0.56a | 1.66 ± 0.14ab | 1.00 ± 0.04b | 17 | 1 |
 G9 | Probable fructokinase-1 | Cs5g22920.1 | 35.11/4.98 | 59.41/5.02 | 718 | 137 | 22 | 0.49 ± 0.10b | 0.58 ± 0.05b | 1.00 ± 0.07a | 20 | 1 |
 G33 | DNA-damage-repair/toleration protein DRT102; ribose-5-phosphate isomerase B | Cs3g11320.1 | 33.52/5.25 | 52.44/5.28 | 279 | 104 | 15 | 0.27 ± 0.06b | 0.79 ± 0.05a | 1.00 ± 0.20a | 14 | 1 |
Protein and amino acid metabolism | ||||||||||||
 G26 | Peptidyl-prolyl cis-trans isomerase FKBP62 | Cs7g16620.3 | 63.83/5.19 | 87.75/5.46 | 344 | 81 | 21 | 0.23 ± 0.02c | 0.79 ± 0.07b | 1.00 ± 0.03a | 19 | 1 |
 G21 | Peptidyl-prolyl cis-trans isomerase CYP38, chloroplastic | Cs2g28260.1 | 48.02/5.00 | 64.55/4.61 | 231 | 80 | 18 | 0.20 ± 0.06c | 0.47 ± 0.04b | 1.00 ± 0.05a | 16 | 1 |
 G30 | Ankyrin repeat domain-containing protein 2 | orange1.1 t02270.1 | 64.18/5.29 | 64.12/4.57 | 261 | 110 | 16 | 0.16 ± 0.04b | 0.72 ± 0.09a | 1.00 ± 0.11a | 15 | 1 |
 G31 | Ankyrin repeat domain-containing protein 2 | orange1.1 t02270.1 | 64.18/5.29 | 65.14/4.51 | 228 | 120 | 13 | 0.30 ± 0.13b | 1.13 ± 0.16a | 1.00 ± 0.21a | 12 | 1 |
 G20 | SKP1-like protein 10 | Cs3g26510.1 | 17.82/4.56 | 33.74/4.58 | 105 | 60 | 11 | 0.16 ± 0.02b | 0.27 ± 0.04b | 1.00 ± 0.13a | 10 | 1 |
 G29 | Subtilisin-like protease SDD1 | Cs1g17350.1 | 82.90/5.86 | 49.48/6.95 | 311 | 107 | 12 | 0.25 ± 0.06b | 0.07 ± 0.01b | 1.00 ± 0.09a | 11 | 1 |
 G24 | 26S protease regulatory subunit 6B homolog | Cs1g08770.1 | 46.49/5.42 | 72.36/5.68 | 425 | 87 | 27 | 1.24 ± 0.12a | 0.42 ± 0.05b | 1.00 ± 0.12a | 25 | 1 |
 G6 | Proline iminopeptidase | Cs8g03250.1 | 44.67/5.70 | 60.15/5.58 | 463 | 116 | 23 | 3.44 ± 0.42a | 2.21 ± 0.41b | 1.00 ± 0.05c | 21 | 1 |
 G25 | Glutamine synthetase cytosolic isozyme | Cs9g05680.1 | 47.86/6.29 | 65.88/5.51 | 181 | 82 | 16 | 0.07 ± 0.02c | 0.61 ± 0.12b | 1.00 ± 0.14a | 15 | 1 |
 G34 | Dihydropyrimidine dehydrogenase [NADP(+)] | orange1.1 t02649.1 | 46.01/6.29 | 66.1/5.75 | 189 | 96 | 16 | 0.28 ± 0.04b | 0.50 ± 0.02b | 1 ± 0.17a | 15 | 1 |
 G27 | Argininosuccinate synthase, chloroplastic | Cs5g07120.1 | 54.38/6.50 | 70.63/5.94 | 371 | 112 | 26 | 2.36 ± 0.13a | 0.90 ± 0.14b | 1.00 ± 0.18b | 24 | 1 |
 G28 | 3-isopropylmalate dehydratase large subunit 1 | Cs5g35290.1 | 55.66/6.68 | 76.63/6.29 | 124 | 72 | 15 | 3.06 ± 0.05a | 1.55 ± 0.12b | 1.00 ± 0.08c | 14 | 1 |
 G22 | Unknown protein DS12 from 2D-PAGE of leaf, chloroplastic; ACT domain-containing protein, putative, expressed | orange1.1 t04488.3 | 26.98/4.94 | 51.32/4.76 | 318 | 89 | 16 | 0.41 ± 0.11b | 1.08 ± 0.26a | 1.00 ± 0.02a | 15 | 1 |
Signal transduction | ||||||||||||
 G32 | Plasma membrane-associated cation-binding protein 1 | Cs2g12010.1 | 23.34/5.03 | 51.44/5.25 | 113 | 37 | 12 | 0.19 ± 0.04b | 0.39 ± 0.04b | 1.00 ± 0.09a | 11 | 1 |
Nucleic acid metabolism | ||||||||||||
 G35 | Adenylosuccinate synthetase 1, chloroplastic | orange1.1 t03395.1 | 53.23/6.40 | 70.64/6.01 | 568 | 101 | 25 | 0.25 ± 0.01b | 0.42 ± 0.09b | 1 ± 0.22a | 23 | 1 |
Lipid metabolism | ||||||||||||
 G37 | Lipase/lipooxygenase, PLAT/LH2 family protein | Cs1g01370.1 | 19.62/4.80 | 22.28/4.22 | 213 | 134 | 7 | 0.15 ± 0.03c | 0.46 ± 0.07b | 1 ± 0.03a | 6 | 1 |
 G38 | Lipase/lipooxygenase, PLAT/LH2 family protein | Cs1g01370.1 | 19.62/4.80 | 44.79/6.6 | 222 | 137 | 7 | 0.33 ± 0.07b | 0.48 ± 0.06b | 1 ± 0.003a | 6 | 1 |
 G14 | Allene oxide cyclase 1, chloroplastic | Cs6g18900.1 | 27.51/8.63 | 34.12/5.73 | 371 | 83 | 13 | 0.19 ± 0.06c | 0.64 ± 0.03b | 1.00 ± 0.11a | 12 | 1 |
Cellular transport | ||||||||||||
 G41 | V-type proton ATPase catalytic subunit A isoform 1 | Cs1g10270.1 | 68.68/5.29 | 59.4/6.96 | 635 | 100 | 40 | 1.50 ± 0.16a | 0.65 ± 0.07b | 1.00 ± 0.08b | 36 | 1 |
Others | ||||||||||||
 G42 | Putative uncharacterized protein Sb01g045410 | Cs4g06170.1 | 26.81/8.90 | 31.73/5.39 | 228 | 82 | 11 | 0.13 ± 0.04b | 0.22 ± 0.03b | 1.00 ± 0.14a | 10 | 1 |
 G43 | Putative uncharacterized protein Sb09g010000 | Cs8g19010.1 | 55.68/7.66 | 80.33/6.05 | 285 | 118 | 20 | 0.46 ± 0.05c | 1.18 ± 0.06a | 1.00 ± 0.02b | 18 | 1 |
 G44 | Uncharacterized protein At2g37660, chloroplastic | Cs6g06590.1 | 36.01/9.43 | 45/6.92 | 485 | 136 | 14 | 0.19 ± 0.03c | 0.58 ± 0.07b | 1.00 ± 0.15a | 13 | 1 |
Unidentified protein spots | ||||||||||||
 G45 | Kinesin-like protein KIF21A | Cs3g17220.3 | 108.17/6.47 | 25.06/4.49 | 52 | / | 28 | 0.27 ± 0.07b | 0.39 ± 0.07b | 1.00 ± 0.25a | 25 | 1 |
 G46 | Actinidain | Cs3g23180.1 | 51.42/5.37 | 52.44/4.62 | 55 | 55 | 2 | 0.35 ± 0.01b | 0.97 ± 0.25a | 1.00 ± 0.13a | 2 | 1 |
 G47 | Peptide methionine sulfoxide reductase B2, chloroplastic | Cs8g03090.1 | 17.59/10.55 | 20.63/5.27 | 51 | 24 | 8 | 0.10 ± 0.009b | 0.34 ± 0.14b | 1.00 ± 0.13a | 7 | 1 |
 G48 | Putative uncharacterized protein | Cs7g03150.1 | 15.56/6.22 | 85.49/5.56 | 55 | 29 | 7 | 0.35 ± 0.06b | 0.85 ± 0.05a | 1.00 ± 0.13a | 6 | 1 |