Spot No.a | Protein identity | Accession No. | Mr (kDa)/pI theor. | Mr (kDa)/pI exp. | Protein score | Peptide ions | NMPb | Ratioc | Coveredd sequence (%) | Charge | ||
---|---|---|---|---|---|---|---|---|---|---|---|---|
pHÂ 2.5 | pHÂ 3 | pHÂ 6 | ||||||||||
Antioxidation and detoxification | ||||||||||||
 S6 | L-ascorbate peroxidase 1, cytosolic | Cs8g17370.1 | 27.57/5.55 | 44.86/5.80 | 584 | 1 26 | 24 | 2.16 ± 0.09a | 1.07 ± 0.12b | 1.00 ± 0.03b | 22 | 1 |
 S4 | L-ascorbate peroxidase 3, peroxisomal | Cs3g19810.2 | 47.33/8.59 | 53.31/5.73 | 573 | 144 | 25 | 2.94 ± 0.80a | 1.11 ± 0.21b | 1.00 ± 0.15b | 23 | 1 |
 S5 | Probable aldo-keto reductase 1 | Cs3g10670.1 | 38.42/5.50 | 62.64/5.68 | 295 | 90 | 15 | 2.41 ± 0.26a | 0.84 ± 0.05b | 1.00 ± 0.17b | 14 | 1 |
 S8 | Isoflavone reductase-like protein | Cs2g16220.1 | 34.25/6.40 | 56.42/6.23 | 155 | 56 | 17 | 2.03 ± 0.29a | 0.80 ± 0.16b | 1.00 ± 0.11b | 15 | 1 |
 S1 | Peroxidase 15 | orange1.1 t02046.1 | 37.43/4.52 | 71.41/4.46 | 178 | 90 | 9 | 0.37 ± 0.07b | 0.45 ± 0.06b | 1.00 ± 0.04a | 8 | 1 |
 S9 | Glutathione S-transferase U19 | Cs5g15190.1 | 25.56/7.56 | 42.77/6.64 | 148 | 71 | 14 | 0.30 ± 0.05b | 0.83 ± 0.10a | 1.00 ± 0.09a | 13 | 1 |
 S10 | Thioredoxin-2 | Cs7g13660.1 | 21.44/8.44 | 21.81/4.87 | 178 | 62 | 7 | 0.47 ± 0.10b | 0.87 ± 0.11a | 1.00 ± 0.06a | 6 | 1 |
Stress response | ||||||||||||
 S11 | Heat shock protein 90–1 | Cs5g03150.1 | 80.52/5.03 | 90.75/5.15 | 186 | 96 | 41 | 1.62 ± 0.19a | 0.53 ± 0.09c | 1.00 ± 0.02b | 37 | 1 |
 S12 | Putative uncharacterized protein Sb02g035950 | Cs1g06050.1 | 27.76/5.56 | 52.74/6.00 | 265 | 87 | 11 | 1.29 ± 0.04a | 0.51 ± 0.14b | 1.00 ± 0.10a | 10 | 1 |
 S13 | Abscisic stress ripening-like protein | Cs3g21500.1 | 20.05/5.75 | 45.68/6.08 | 411 | 92 | 14 | 0.20 ± 0.05c | 1.59 ± 0.11a | 1.00 ± 0.06b | 13 | 1 |
 S17 | Thiamine thiazole synthase 1, chloroplastic | Cs4g11090.1 | 37.60/5.40 | 53.52/5.22 | 304 | 95 | 15 | 1.64 ± 0.12a | 0.75 ± 0.02b | 1.00 ± 0.18b | 14 | 1 |
 S39 | S-norcoclaurine synthase; Pathogenesis-related (PR)-10-related norcoclaurine synthase-like protein | Cs6g03210.1 | 17.29/4.89 | 28.52/5.12 | 485 | 90 | 17 | 4.45 ± 0.27a | 2.23 ± 0.16b | 1.00 ± 0.13c | 15 | 1 |
Carbohydrate and energy metabolism | ||||||||||||
 S14 | Chlorophyll a-b binding protein 215 | Cs1g06360.1 | 28.93/5.13 | 41.21/4.90 | 71 | 56 | 6 | 2.22 ± 0.10a | 0.46 ± 0.03c | 1.00 ± 0.16b | 5 | 1 |
 S20 | Chlorophyll a-b binding protein 4, chloroplastic | Cs3g06180.1 | 29.52/6.84 | 40.18/5.42 | 167 | 88 | 11 | 0.13 ± 0.02c | 0.71 ± 0.07b | 1.00 ± 0.11a | 10 | 1 |
 S16 | Oxygen-evolving enhancer protein 1–1, chloroplastic | Cs1g23450.1 | 35.38/5.83 | 49.12/5.09 | 371 | 110 | 14 | 2.20 ± 0.59a | 0.82 ± 0.11b | 1.00 ± 0.02b | 13 | 1 |
 S19 | Oxygen-evolving enhancer protein 1–1, chloroplastic | Cs1g23450.1 | 35.38/5.83 | 48.88/5.44 | 523 | 135 | 19 | 2.67 ± 0.76a | 0.99 ± 0.19b | 1.00 ± 0.03b | 17 | 1 |
 S18e | Ribulose bisphosphate carboxylase/oxygenase activase 1, chloroplastic | Cs7g31800.4 | 50.90/5.33 | 68.00/5.12 | 534 | 91 | 23 | 0.30 ± 0.06b | 0.44 ± 0.06b | 1.00 ± 0.09a | 21 | 1 |
 S21 | Ribulose bisphosphate carboxylase/oxygenase activase 1, chloroplastic | Cs7g31800.3 | 46.96/5.94 | 65.74/5.36 | 511 | 109 | 20 | 0.21 ± 0.02b | 0.91 ± 0.02a | 1.00 ± 0.08a | 18 | 1 |
 S22 | Rubisco subunit binding-protein β-2 subunit; Chaperonin 60 subunit β 1 | Cs9g03300.1 | 64.78/5.85 | 81.19/5.36 | 798 | 107 | 39 | 0.33 ± 0.04b | 1.10 ± 0.10a | 1.00 ± 0.13a | 35 | 1 |
 S35 | Ferredoxin-NADP reductase, leaf isozyme, chloroplastic | Cs1g25510.1 | 40.48/8.68 | 55.49/6.40 | 258 | 80 | 22 | 0.45 ± 0.04c | 0.65 ± 0.08b | 1.00 ± 0.02a | 20 | 1 |
 S37 | Ferredoxin-NADP reductase, leaf isozyme, chloroplastic | Cs1g25510.4 | 40.48/8.68 | 55.29/6.70 | 240 | 82 | 21 | 0.45 ± 0.03b | 0.91 ± 0.11a | 1.00 ± 0.13a | 19 | 1 |
 S27 | Phosphoglycerate kinase 1, chloroplastic | orange1.1 t03280.1 | 49.45/8.20 | 67.19/5.91 | 567 | 95 | 25 | 0.25 ± 0.10b | 0.87 ± 0.06a | 1.00 ± 0.11a | 23 | 1 |
 S29 | Phosphoglycerate kinase 1, chloroplastic | orange1.1 t03280.1 | 49.45/8.20 | 80.46/5.95 | 346 | 92 | 13 | 0.06 ± 0.02b | 0.95 ± 0.10a | 1.00 ± 0.14a | 12 | 1 |
 S23 | 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, chloroplastic | Cs3g01420.1 | 32.59/8.29 | 47.43/5.72 | 415 | 121 | 14 | 0.31 ± 0.04b | 0.80 ± 0.08a | 1.00 ± 0.15a | 13 | 1 |
 S24 | Succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial | Cs5g29390.1 | 45.26/5.98 | 63.82/5.62 | 286 | 78 | 27 | 0.52 ± 0.13b | 1.10 ± 0.06a | 1.00 ± 0.06a | 25 | 1 |
 S28 | Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial | Cs1g17930.1 | 59.37/8.43 | 77.39/5.80 | 390 | 94 | 23 | 0.30 ± 0.06c | 0.77 ± 0.04b | 1.00 ± 0.05a | 21 | 1 |
 S32 | Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex 1, mitochondrial | Cs2g21190.1 | 51.06/9.07 | 72.17/6.03 | 181 | 135 | 11 | 0.19 ± 0.04b | 0.86 ± 0.04a | 1.00 ± 0.07a | 10 | 1 |
 S3 | NADH dehydrogenase [ubiquinone] iron-sulfur protein 8-A, mitochondrial | Cs8g06410.1 | 26.11/5.68 | 42.59/5.12 | 238 | 48 | 20 | 2.58 ± 0.27a | 1.10 ± 0.10b | 1.00 ± 0.09b | 18 | 1 |
 S26 | ATP synthase gamma chain 1, chloroplastic | Cs2g03080.1 | 40.62/6.08 | 59.62/5.82 | 208 | 103 | 10 | 0.19 ± 0.04c | 0.44 ± 0.06b | 1.00 ± 0.06a | 9 | 1 |
 S30 | Probable ATP synthase 24 kDa subunit, mitochondrial | Cs1g04030.1 | 27.52/8.90 | 46.81/6.24 | 368 | 96 | 22 | 0.22 ± 0.06b | 0.55 ± 0.11b | 1.00 ± 0.14a | 20 | 1 |
 S36 | Probable ATP synthase 24 kDa subunit, mitochondrial | Cs1g04030.1 | 27.52/8.90 | 45.64/6.86 | 543 | 102 | 27 | 0.29 ± 0.07b | 0.68 ± 0.08ab | 1.00 ± 0.24a | 25 | 1 |
 S47 | DNA-damage-repair/toleration protein DRT102; ribose-5-phosphate isomerase B | Cs3g11320.1 | 33.52/5.25 | 53.47/5.39 | 393 | 85 | 19 | 0.44 ± 0.07b | 1.02 ± 0.10a | 1.00 ± 0.16a | 17 | 1 |
Protein and amino acid metabolism | ||||||||||||
 S40 | Peptidyl-prolyl cis-trans isomerase CYP37, chloroplastic | Cs1g06710.1 | 50.39/6.42 | 60.41/5.11 | 579 | 139 | 21 | 3.06 ± 0.91a | 1.36 ± 0.22ab | 1.00 ± 0.11b | 19 | 1 |
 S43 | T-complex protein 1 subunit beta | Cs3g26890.2 | 57.19/5.56 | 79.99/5.91 | 226 | 73 | 22 | 0.30 ± 0.13b | 1.04 ± 0.05a | 1.00 ± 0.19a | 20 | 1 |
 S41 | Proteasome subunit beta type-6-A like protein | Cs7g07630.1 | 25.57/5.21 | 40.62/5.73 | 254 | 86 | 12 | 0.23 ± 0.01c | 0.68 ± 0.04b | 1.00 ± 0.10a | 11 | 1 |
 S44 | 26S proteasome non-ATPase regulatory subunit 11A | Cs4g04180.1 | 47.05/5.79 | 70.34/6.16 | 367 | 97 | 24 | 0.22 ± 0.028b | 0.83 ± 0.091a | 1.00 ± 0.06a | 22 | 1 |
 S50 | Unknown protein DS12 from 2D-PAGE of leaf, chloroplastic; ACT domain-containing protein, putative, expressed | orange1.1 t04488.1 | 30.69/5.59 | 51.57/4.75 | 333 | 105 | 15 | 0.35 ± 0.07b | 0.89 ± 0.02a | 1.00 ± 0.04a | 14 | 1 |
 S51 | Kynurenine formamidase | Cs8g05140.1 | 30.62/5.32 | 55.58/5.48 | 270 | 75 | 13 | 0.44 ± 0.01b | 1.04 ± 0.03a | 1.00 ± 0.08a | 12 | 1 |
Cell wall and cytoskeleton | ||||||||||||
 S45 | Tubulin alpha-1 chain | Cs9g03120.1 | 49.75/4.99 | 73.13/5.41 | 487 | 85 | 20 | 0.40 ± 0.05b | 0.93 ± 0.12a | 1.00 ± 0.13a | 18 | 1 |
 S46 | Caffeic acid 3-O-methyltransferase 1 | orange1.1 t05216.1 | 29.98/5.73 | 63.37/6.19 | 194 | 66 | 11 | 0.53 ± 0.09b | 1.18 ± 0.04a | 1.00 ± 0.04a | 10 | 1 |
Nucleic acid metabolism | ||||||||||||
 S48 | Hypoxanthine-guanine phosphoribosyltransferase | Cs3g21990.1 | 20.48/5.54 | 38.60/5.61 | 143 | 94 | 8 | 0.41 ± 0.03b | 1.07 ± 0.17a | 1.00 ± 0.08a | 7 | 1 |
Lipid metabolism | ||||||||||||
 S2 | Plastid lipid-associated protein 2, chloroplastic; Chromoplast-specific carotenoid-associated protein, chromoplast | Cs2g02520.1 | 43.18/6.08 | 50.48/4.67 | 503 | 92 | 24 | 1.68 ± 0.09a | 0.79 ± 0.18b | 1.00 ± 0.02b | 22 | 1 |
 S7 | Epoxide hydrolase 4 | Cs2g06360.1 | 36.94/5.90 | 59.20/6.19 | 352 | 73 | 21 | 2.51 ± 0.27a | 0.90 ± 0.10b | 1.00 ± 0.18b | 19 | 1 |
 S31 | Cinnamoyl-CoA reductase 1 | Cs8g20610.1 | 35.48/5.57 | 55.34/6.06 | 220 | 77 | 14 | 0.31 ± 0.02b | 0.66 ± 0.21ab | 1.00 ± 0.12a | 13 | 1 |
Cellular transport | ||||||||||||
 S15 | Ferritin-1, chloroplastic | Cs6g09150.2 | 28.97/5.46 | 43.81/4.92 | 88 | 64 | 8 | 1.97 ± 0.34a | 0.69 ± 0.09b | 1.00 ± 0.11b | 7 | 1 |
Signal transduction | ||||||||||||
 S38 | 14–3-3-like protein GF14 kappa; General regulatory factor 8 | Cs1g20220.1 | 27.69/4.87 | 45.64/4.90 | 339 | 106 | 20 | 4.71 ± 0.32a | 1.20 ± 0.15b | 1.00 ± 0.06b | 18 | 1 |
Others | ||||||||||||
 S52 | Putative uncharacterized protein Sb02g003450 | Cs5g09380.2 | 27.69/7.84 | 45.27/5.58 | 193 | 60 | 14 | 0.38 ± 0.11b | 1.00 ± 0.09a | 1.00 ± 0.12a | 13 | 1 |
 S42 | Putative uncharacterized protein Sb09g010000 | Cs8g19010.1 | 55.68/7.66 | 79.38/5.66 | 248 | 102 | 19 | 0.19 ± 0.06b | 0.73 ± 0.13a | 1.00 ± 0.22a | 17 | 1 |
 S34 | Flavoprotein WrbA | Cs4g11860.1 | 22.29/5.75 | 40.45/6.51 | 256 | 136 | 10 | 0.44 ± 0.05b | 0.67 ± 0.07ab | 1.00 ± 0.14a | 9 | 1 |
 S49 | NAD(P)H-dependent 6′-deoxychalcone synthase | orange1.1 t00001.2 | 67.34/9.06 | 57.67/6.61 | 247 | 100 | 20 | 0.46 ± 0.07b | 0.67 ± 0.11b | 1.00 ± 0.07a | 18 | 1 |
Unidentified protein spots | ||||||||||||
 S33 | Probable phosphoglucomutase, cytoplasmic 1 | orange1.1 t05474.1 | 16.20/5.32 | 84.77/6.25 | 100 | 91 | 4 | 0.40 ± 0.05b | 0.77 ± 0.08ab | 1.00 ± 0.17a | 4 | 1 |
 S25 | Glucose-6-phosphate 1-dehydrogenase 1, chloroplastic | Cs7g11110.1 | 66.95/7.68 | 25.37/5.76 | 65 | 23 | 21 | 0.46 ± 0.04b | 1.34 ± 0.08a | 1.00 ± 0.23b | 19 | 1 |
 S53 | Endo-1,3;1,4-beta-D-glucanase | Cs9g05910.3 | 28.00/7.07 | 43.84/5.09 | 43 | / | 12 | 3.32 ± 0.29a | 1.02 ± 0.07b | 1.00 ± 0.09b | 11 | 1 |
 S54 | E3 ubiquitin-protein ligase MARCH9 | Cs9g05100.7 | 32.53/8.56 | 16.93/6.1 | 44 | / | 12 | 4.26 ± 0.19a | 0.45 ± 0.02b | 1.00 ± 0.18b | 11 | 1 |
 S55 | Cytochrome b6-f complex iron-sulfur subunit, chloroplastic | Cs2g22650.3 | 22.32/8.76 | 30.95/8.76 | 52 | 36 | 6 | 0.33 ± 0.04c | 0.75 ± 0.05b | 1.00 ± 0.09a | 5 | 1 |