Fig. 1

MYB1::3xFLAG transcripts and protein detection. a – Western Blot of MYB1::3xFLAG proteins in tECL. Total proteins from non-transformed ECL (ntECL) were used as negative control. b – MYB1::3xFLAG transcripts levels from six transformed embryogenic cell lines (tECL) clusters (52.1–52.6) quantified by RT-qPCR and normalised against non-transformed embryogenic cell lines (ntECL)(81). Mean and standard errors of three independent experiments are shown. c – MYB1::3xFLAG protein nuclear detection by fluorescence microscopy in tECL. Nucleus magnification is shown for tECL and ntECL. Bars = 5 μm