Fig. 1

Seeds of ERF8 knockdown (erf8–1) and overexpression lines (ERF8-OE) exhibit altered ABA sensitivity. Wildtype Ws-2 and erf8–1 knockdown seeds were sown on 0.5 X MS agar plates with or without 0.8 μM ABA. (a) Representative seed populations under 24 h light exposure at 5 days post stratification (dps). (b) Average percentage of germination of two replicates at 5 dps. Graph depicts the proportion of seeds that were not germinated (NG), exhibited radicle (R) or cotyledon(s) emergence (C). (c) Two independent lines of DEX-inducible ERF8-OE seeds germinated in the presence of 1 μM ABA with or without 50 nM DEX. (d) Two independent lines of DEX-inducible ERF8-OE seeds were germinated in the presence of 1 μM ABA with or without 50 nM DEX. Average percentage of germination at 14 dps.*p ≤ 0.01