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Table 1 Chloroplast proteins over-accumulated in the clpc1 mutant that were previously identified [11] in clpc1 mutant (1a) or clpp6 antisense line (1b) as putative targets of CLPP

From: The caseinolytic protease complex component CLPC1 in Arabidopsis maintains proteome and RNA homeostasis in chloroplasts

  1st batch
4-week-old (LD)
2nd batch (2 biological replicates)
2-week-old (LD)
Nishimura et al (2013)
6-week-old (SD)
symbol accession clpc1/WS ΔN/WS CP/WS clpc1/WS ΔN/WS CP/WS clpc1–1/wt
1a. Previous identified misregulated proteins in clpcl mutant
 RH3 AT5G26742.1 8.3 ± 0.8* 3.0 ± 0.3 0.7 ± 0.1 2.6 ± 0.1* 2.0 ± 0.1 0.8 ± 0.1 1.0
 CPN60A AT2G28000.1 4.9 ± 0.2 3.6 ± 0.1 0.8 ± 0.1 1.9 ± 0.1 1.9 ± 0.1 0.7 ± 0.2 1.4
 TCP-1 AT3G13470.1 4.7 ± 0.2 3.5 ± 0.1 0.8 ± 0.1 1.8 ± 0.0 2.0 ± 0.0 0.6 ± 0.1 1.7
 HSP90.5 AT2G04030.1 4.0 ± 0.2 2.7 ± 0.2 0.9 ± 0.1 1.6 ± 0.1 1.5 ± 0.1 0.6 ± 0.0 1.6
 cpHsc70–2 AT5G49910.1 3.7 ± 0.1* 2.8 ± 0.1 0.8 ± 0.1 1.8 ± 0.2* 1.8 ± 0.1 0.5 ± 0.0 1.1
 cpHsc70–1 AT4G24280.1 3.7 ± 0.1* 2.9 ± 0.1 0.8 ± 0.1 1.8 ± 0.3* 1.9 ± 0.2 0.5 ± 0.0 1.0
 RNA-binding AT2G37220.1 3.5 ± 0.3 2.2 ± 0.2 0.8 ± 0.1 1.8 ± 0.0 1.9 ± 0.1 0.6 ± 0.1 2.3
 EF-Ts AT4G29060.1 3.5 ± 0.2* 2.2 ± 0.2 0.8 ± 0.1 1.4 ± 0.2 1.3 ± 0.1 0.6 ± 0.1 0.9
 CPN21 AT5G20720.1 2.8 ± 0.2 3.1 ± 0.1 0.7 ± 0.1 1.8 ± 0.3 1.7 ± 0.2 0.5 ± 0.0 2.1
 ATTIC110 AT1G06950.1 2.6 ± 0.2* 2.0 ± 0.1 0.9 ± 0.1 1.3 ± 0.0* 1.4 ± 0.0 0.7 ± 0.1 0.0
 lipoxygenase 2 AT3G45140.1 1.5 ± 0.1* 1.0 ± 0.1 1.3 ± 0.1 1.5 ± 0.0* 1.6 ± 0.0 1.5 ± 0.2 1.0
 AtcpSecA AT4G01800.1 1.4 ± 0.1 1.4 ± 0.1 0.9 ± 0.1 1.0 ± 0.1 1.1 ± 0.1 0.6 ± 0.1 1.4
1b. Clpc1 mutant mis-regulated CLPP6 knocking down line identified targets  
 RH3 AT5G26742.1 8.3 ± 0.8* 3.0 ± 0.3 0.7 ± 0.1 2.6 ± 0.1* 2.0 ± 0.1 0.8 ± 0.1 1.0
 HSP90.5 AT2G04030.1 4.0 ± 0.2 2.7 ± 0.2 0.9 ± 0.1 1.6 ± 0.1 1.5 ± 0.1 0.6 ± 0.0 1.6
 EF-Ts AT4G29060.1 3.5 ± 0.2* 2.2 ± 0.2 0.8 ± 0.1 1.4 ± 0.2 1.3 ± 0.1 0.6 ± 0.1 0.9
 FBA2 AT4G38970.1 2.9 ± 0.1* 2.0 ± 0.1 0.7 ± 0.1 1.4 ± 0.3 1.2 ± 0.1 0.5 ± 0.1 0.9
 ATNDPK2 AT5G63310.1 2.8 ± 0.6 2.3 ± 0.5 0.8 ± 0.1 1.3 ± 0.0 1.3 ± 0.0 0.6 ± 0.1 1.2
 Ribose 5-phospha AT3G04790.1 2.7 ± 0.3 2.0 ± 0.3 0.8 ± 0.1 1.1 ± 0.0 1.1 ± 0.1 0.6 ± 0.2 1.0
 ROC4 AT3G62030.1 1.8 ± 0.1 1.5 ± 0.1 0.9 ± 0.1 1.1 ± 0.1 1.3 ± 0.1 0.3 ± 0.0 0.9
  1. WS: wild type; clpc1: the clpc1 mutant; ΔN, N-terminal (1–93 amino acid) deleted CLPC1 complementary line; CP, full-length CLPC1 complementary line, LD, long-day; SD, short-day
  2. The first batch dataset was from 4-week-old seedlings with three technical replicates. The second batch was from 2-week-old seedlings with two biological replicates. Each biological replicate included 3 technical replicates
  3. Data are means and standard errors of protein abundance relative to the wild type (WS)
  4. Data from Nishimura et al... (2013) in [28]
  5. Proteins marked with * are significantly (p < 0.05) over- or under-accumulated in the clpc1 mutant in the current study that were not significantly altered or not detected in a previous study (Nishimura et al..... 2013)