Fig. 6

Expression and phenotypic analyses of GhSIF1 under salt treatment and in VIGS treat cotton plants. (a) Cotton (G. hirsutum) seeds germinated on ½ MS medium were transferred to ½ MS with or without 300 mM NaCl medium. Ten days later, leaves and roots were collected for real-time PCR analysis. GhActin2 was used as the reference gene. (b) 10 days old cotton plants (G. hirsutum) with two cotyledon leaves were infiltrated with TRV1 and empty TRV2 (as control) or TRV2-GhSIF1 (targeting GhSIF1 mRNA). Ten days later, leaf samples were collected for real-time PCR analysis. GhActin2 was used as the reference gene. Data shown are an average of three technical replicates for two independent biological replicates. Error bars represent S.D. (n = 6). The statistically significant difference was determined by t-test. P < 0.05 was marked as *. P < 0.01 was marked as **. Pictures were taken (c) before salt treatment and (d) 18 days after salt treatment