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Fig. 8 | BMC Plant Biology

Fig. 8

From: Jasmonate promotes auxin-induced adventitious rooting in dark-grown Arabidopsis thaliana seedlings and stem thin cell layers by a cross-talk with ethylene signalling and a modulation of xylogenesis

Fig. 8

ARF6::GUS and ARF17::GUS expression patterns in IBA + Kin-TCLs cultured with or without MeJA. (a-h) Localization of the GUS signal in ARF6::GUS (a-d) and ARF17::GUS TCLs (e-h) cultured under darkness for 15 days in the absence of MeJA (0 μM MeJA) (a, c) and in the presence of 0.01 μM MeJA (b, d-e, g) or of 0.1 μM MeJA (f, h). (a) GUS signal in the ARPs and in the apex of elongated ARs. (b) TCL showing the same ARF6::GUS expression as in (a). The high presence of the signal is in accordance with the production of ARPs/ARs higher in (b) than in (a). (c-d) Localization of the GUS signal in the apical part of the ARPs. (e-f) ARF17::GUS expression absent in the ARs, but present along the explant. (g) ARF17::GUS expression localized in the cells differentiating into xylary elements, as magnified in the Inset. (h) ARF17 GUS signal present in the strands of cells differentiating into xylary elements, but absent in the ARPs. (a-b, e-f) Stereomicroscope images, (c-d, g-h) Longitudinal radial sections of TCLs observed under LM. (Images from the first replicate). Bars = 50 μm (Inset in g), 100 μm (d), 200 μm (c, f-h), 500 μm (e), 1 mm (a, b)

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BMC Plant Biology

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