Skip to main content
Fig. 7 | BMC Plant Biology

Fig. 7

From: Jasmonate promotes auxin-induced adventitious rooting in dark-grown Arabidopsis thaliana seedlings and stem thin cell layers by a cross-talk with ethylene signalling and a modulation of xylogenesis

Fig. 7

Immunolocalization of JA/JA-Ile in Ws and opr3 IBA + Kin-cultured TCLs. (a-h) Immunocytochemical detection of JA/JA-Ile in Ws (a-d, g-h) and in the JA-deficient mutant opr3 (e-f) longitudinal radial sections of TCLs, at days 8 and 15 of culture under darkness in the absence (a, e) or in the presence of 0.01 μM MeJA (b-d, f) or 10 μM MeJA (g-h). (a) Cells of a meristemoid showing the green epifluorescence signal of JA/JA-Ile immunodetection (day 8). (b) Cells of a meristematic clump showing an epifluorescence signal more intense than in (a) (day 8). (c) Detail of an AR tip (view in longitudinal section) showing apical cells with the signal (day 15). (d) Detail of an AR tip (view in transection) showing cells with the signal (day 15). (e) opr3 ARP cells with no signal (arrow) in a TCL cultured without exogenous MeJA (day 15). (f) Faint signal in an opr3 ARP (arrow) of a TCL cultured with 0.01 μM MeJA (day 15). (g) Weak signal (arrows) inside the cells of a differentiating xylary clump (day 15). (h) Weak signal (arrow) inside immature de novo formed xylary cells (day 15). (Images from the first replicate). Bars = 20 μm (a, b, g, h), 40 μm (c, d), 60 μm (e, f)

Back to article page

BMC Plant Biology

ISSN: 1471-2229

Contact us