Fig. 10

AR-formation in IBA + Kin-grown ein3eil1 seedlings and TCLs, and TCLs of JA-mutants, with/without ACC-treatment. (a) Mean AR density, i.e. mean AR/ARP number per cm of hypocotyl (±SE), in Col-0 (WT) and ein3eil1 seedlings grown under darkness in the presence/absence of 0.1 μM ACC, at 22 DAS. (b) Mean AR/ARP number per explant (±SE) in TCLs excised from ein3eil1, dde2–2, coi1–16 mutant plants, and their WTs (Col-0, Col and Col-gl1, respectively), after 15 days of culture under darkness in the presence/absence of 0.1 μM ACC. (c-d) Histological images of Col-0 TCLs cultured in the absence of ACC (c) and with 0.1 μM ACC (d), at day 15. (a-b) Significant differences between treatments within the same genotype, or between each mutant and its WT under the same treatment, at least at P < 0.05 level, are indicated by different letters. The same letter within the same genotype, and between each mutant and its WT, shows no significant difference. Further statistical details are described in the text. N = 30 (a), N = 60 (b) (First replicate). (c) Section area of a TCL showing numerous ARs and a limited number of de novo formed xylary cells in the explant. (d) Detail of an ACC-cultured TCL showing a conspicuous differentiation of single, or grouped in clumps or strands, xylary cells (shown by arrows). Note that the lignified cell walls are in blue-green colour. Toluidine blue staining. (Images from the first replicate). Bars = 200 μm