Fig. 6From: Cytosolic GAPDH as a redox-dependent regulator of energy metabolismBinding of Trx-h3 to GapC1. Binding kinetics of Trx-h3 to immobilized GapC1 were monitored by RIf spectroscopy. The experiment was performed in 20Â mM HBS-buffer (100Â mM NaCl, 0.01% Triton X-100, pHÂ 7.5) supplemented with 2Â mM DTT. The GapC1 protein was immobilized via NHS. Association and dissociation reactions were recorded at different Trx-h3 concentrationsBack to article page