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Fig. 4 | BMC Plant Biology

Fig. 4

From: Cytosolic GAPDH as a redox-dependent regulator of energy metabolism

Fig. 4

Redox-dependent distribution of GapC between cytosol and nucleus. For quantification of nuclear localization, mesophyll protoplasts were transiently transformed with GapC-mEGFP fusions of the isoforms GapC1 (a), and GapC2 (b), and their corresponding single (C156S and C160S) and double (C156S/C160S) cysteine mutants. The subcellular localization was determined after the treatment with different redox reagents (10 mM DTT; 0.2 mM H2O2 or 0.05 mM GSNO). (n ≥ 50 analyzed protoplasts. The sum of cytosolic and nuclear mEGFP-signal corresponds to 100% for each experiment; mean ± SD)

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