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Fig. 1 | BMC Plant Biology

Fig. 1

From: Cytosolic GAPDH as a redox-dependent regulator of energy metabolism

Fig. 1

Effect of the redox-state on colocalization of GapC1 with mitochondria in Arabidopsis protoplasts. Protoplasts were transiently transformed with p35S-GapC1-mEGFP. Mitochondrial staining was performed by incubating the protoplasts in 500 nM MitoTracker® Red FM for 40 min. The protoplasts were pre-treated with 10 mM DTT or 0.2 mM H2O2 for 30 min to establish defined oxidizing and reducing conditions. Fluorescence signals are displayed in false colors: GapC1-mEGFP (cyan), MitoTracker® Red FM (magenta); chlorophyll autofluorescence (yellow). To estimate a tendency for the occurrence of colocalization, protoplasts (n ≥ 50) wereinspected. A representative image is shown for each condition.and Colocalization events are visualized in the merged picture as white regions. The scale bar is set to 10 μm

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