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Fig. 3 | BMC Plant Biology

Fig. 3

From: Identification of a G2-like transcription factor, OsPHL3, functions as a negative regulator of flowering in rice by co-expression and reverse genetic analysis

Fig. 3

Subcellular localization and transactivation analysis of OsPHL3. a Subcellular localization of OsPHL3-GFP fusion protein under the control of CaMV35S promoter. b Bright field. c The nuclear marker mCherry-VirD2NLS vector (mCherry). d Merged image. Bar = 2um from A to D. (E) OsPHL3 full-length coding region, N-terminal region containing Myb-DNA binding domain (1–100 amino acids) or the C-terminal region containing CC binding domain (101–251 amino acids) were individually inserted into pBD-GAL4 DNA binding-domain contained plasmid. The empty pBD vector was used as control. The expression of OsPHL3 coding region and OsPHL3-C terminal showed resistance to toxic drug Aureobasidin A

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