Fig. 1

Cellulase pre-treatment of A. thaliana seedlings induces resistance to subsequent alamethicin permeabilisation. a Seedlings were grown in H₂O for 4 days. They were then transferred to cellulase in H₂O or only H₂O (control), for 4 h, then washed with H₂O and incubated for 10 min with 20 μg ml^− 1 alamethicin, and then for 45 s with PrI and quickly washed with H₂O. Pictures were taken after washing and transfer of seedlings to new medium without alamethicin, using a dissection microscope with or without red filter and with UV excitation. Bar corresponds to 5 mm. The images show one representative replicate out of three. b Seedlings were grown in ½ MS for 10 days. They were incubated in cellulase in mannitol or only mannitol (control), for 2 h, and then for 15 min with various concentrations of alamethicin, where PrI was included the last 5 min. Pictures were taken directly using a fluorescence microscope equipped with a G2A filter. Bars correspond to 100 μm. The images show one representative replicate out of three