Fig. 7

Structure of the CiFT co-expression vector with antisense CitRKD1-mg2 (a) and gene expression of CitRKD1 in leaves and flowers of control ‘Hamlin’ sweet orange (CNT) and transgenic sweet orange by RT-PCR (b). The transcription level of endogenous CitRKD1-org is investigated using CitRKD1-org specific primers. Transgenic sweet orange bearing fruits, seeds of control and transgenic ‘Hamlin’ sweet oranges after removing their seed coats are photographed (c). Only one independent line of regenerated transgenic sweet orange was obtained out of 1274 Agrobacterium infected epicotyl segments which are generally sufficient number to obtained plural independent transgenic lines, probably owing to deleterious effects of transgene