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Fig. 7 | BMC Plant Biology

Fig. 7

From: Comprehensive analysis of NAC transcription factors uncovers their roles during fiber development and stress response in cotton

Fig. 7

Transactivation analysis of GhNAC genes. a The transcriptional activity of selected NAC proteins in cotton embryogenic callus protoplasts. Renilla LUC was used as the internal control. Three independent experiments were performed. Asterisks indicate significant differences, ** P ≤ 0.01, student’s t-test. The LUC/Renilla Ratio of each gene compared with control (GAL4DB), respectively. b Transactivation assay of NAC proteins in yeast strain Y2H. The transactivation activities were determined by the growth on SD medium without Tryptophane (Trp) and added X-α-Gal (SD-Trp + X-α-Gal). The photograph was taken after 36 h incubation. Positive control: pGBKT7–53; negative control: pGBKT7-Lam. c Transactivation assay of GhNAC158. Full: the full length of GhNAC158, BD: NAC domain (Binding Domain) of GhNAC158, AD: the C terminal (Activation Domain) of GhNAC158. d Transactivation assay of GhNAC157. TM: the transmembrane motif (TM) deleted form of GhNAC157

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