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Fig. 10 | BMC Plant Biology

Fig. 10

From: Genome-wide analysis of genes encoding core components of the ubiquitin system in soybean (Glycine max) reveals a potential role for ubiquitination in host immunity against soybean cyst nematode

Fig. 10

The differential expression patterns of soybean UBS genes revealed by RNA-seq analysis are validated by real-time quantitative PCR (qPCR). Roots from three-week-old soybean Williams 82 (Wm 82) plants were independently inoculated with two soybean cyst nematode (SCN, Heterodera glycines Ichinohe) populations, race 155 (HG Type 2.5.7) and race 117 (HG Type 1.2.3.5.6.7) for one week, and then collected for RNA extraction. The root of uninoculated Wm82 plants was used as control. Real time qPCR experiments were performed using the soybean EF1a gene (GmEF1a) as the internal control and mean values ± SD of three independent experiments were shown. Statistically significant differences were analyzed using Student’s t-test (* p < 0.05, ** p < 0.01)

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BMC Plant Biology

ISSN: 1471-2229

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