Fig. 2
From: Phosphorylation of Arabidopsis SINA2 by CDKG1 affects its ubiquitin ligase activity
E3 ubiquitin ligase activity of SINA2. a In vitro auto-ubiquitination activity assays were performed in the presence of human E1, human E2 (UbcH5a) and ubiquitin. Samples were resolved by SDS-PAGE on 10% gels and detected by immunoblot analysis using anti-Ub. b Various human E2 enzymes were tested for ubiquitin-conjugating activity in the presence of His-SINA2 and only assays containing UbcH5a showed positive results