Expression levels of BrpHTT genes after heat-shock treatment and the phenotype of HTT2-overexpressing transgenic plants. (a) Expression levels of BrpHTT genes after heat-shock treatment. (b) rHTT2 RNA sequences complementary to siR255. (c) HTT2–2 and Bre plants at the folding stage. (d) Quantitative real-time polymerase chain reaction (qRT-PCR) results showing the relative expression levels of HTT2 in Bre and HTT2–2 plants. (e) qRT-PCR results showing the relative expression of HTT2 in HTT2–2 plants after heat treatment at different temperatures. Error bars represent standard deviation (SD). Differences were considered significant (*) if p < 0.05 (Student’s t-test) and highly significant (**) if p < 0.01 (Student’s t-test).