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Fig. 5 | BMC Plant Biology

Fig. 5

From: An efficient and improved method for virus-induced gene silencing in sorghum

Fig. 5

In situ hybridization shows the uneven spread of BMV virus in sorghum. In situ hybridization probe was designed to hybridize the RNA encoding BMV coat protein. The purple pigment (red arrows) indicates the presence of the virus near veins of the infected leaf. The absence of purple color in the uninfected control sorghum plant indicate a lack of virus. The image was taken with a Nikon TE300 microscope. a Sorghum leaf segment observed by the 10X magnification of the Nikon TE300 microscope. b The sorghum leaf segment observed by the 20X magnification of the microscope. A similar pattern was seen in two biological replicates

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