Fig. 2
From: An efficient and improved method for virus-induced gene silencing in sorghum

Sorghum plants incubated at 18 °C enhance BMV infection. a BMV::GFP infection showed more viral symptoms (bleaching/yellowing) in maize and barley compared to sorghum at 22 °C. b Virus with GFP and sorghum gene fragments: ChlH, or PDS, were grown in N. benthamiana, and sap was isolated and rubbed on Chenopodium, barley, and sorghum plants. BMV was able to infect Chenopodium and barley at 22 °C and sorghum at 18 °C.