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Table 2 Mean yield in cotyledonary somatic embryos (SEs) of Douglas-fir from 2ry and 3ry embryogenic lines

From: Repetitive somatic embryogenesis induced cytological and proteomic changes in embryogenic lines of Pseudotsuga menziesii [Mirb.]

Line

Mean no. of SEs g−1 f.w.

Secondary

 SD4–8

2401

± 534a

Tertiary

 SD4–8-1

3344

± 1274ab

 SD4–8-2

4160

± 931b

 SD4–8-3

4258

± 829b

  1. Values are means of 3 biological and 5–6 technical replicates ±95% confidence limits. Significant differences (p < 0.05) in multiple comparisons of means are indicated by different letters