Table 1 Effects of sucrose on polyphenol accumulation in different tissues of tea plantlets after 14d treatment using UPLC-QQQ-MS/MS
Compound | Control | Suc | ratio | Control | Suc | ratio | Control | Suc | ratio | Control | Suc | ratio |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
bud | bud | Â | 3rd leaf | 3rd leaf | Â | up-stem | up-stem | down-stem | down-stem | |||
Phenolic acid (mg/g) | ||||||||||||
 Quinic acid | 44.21 ± 2.01 | 86.06 ± 4.05 | 1.95 | 6.55 ± 0.23 | 7.45 ± 0.35 | 1.14 | 39.43 ± 1.89 | 40.19 ± 1.70 | 1.02 | 3.72 ± 0.15 | 6.19 ± 0.29 | 1.67 |
Gallic acid derivatives | ||||||||||||
  β-glucogallin | 9.42 ± 0.41 | 1.47 ± 0.11 | 0.16 | 0.90 ± 0.05 | 0.97 ± 0.05 | 1.08 | 2.83 ± 0.12 | 0.81 ± 0.03 | 0.29 | 0.02 ± 0.00 | 0.01 ± 0.00 | 0.73 |
  galloyl acid | 0.38 ± 0.01 | 0.36 ± 0.02 | 0.95 | 0.08 ± 0.00 | 0.10 ± 0.01 | 1.19 | 0.25 ± 0.01 | 0.16 ± 0.01 | 0.65 | 0.20 ± 0.01 | 0.03 ± 0.00 | 0.13 |
  galloylquinic acid | 14.09 ± 0.9 | 13.29 ± 0.7 | 0.94 | 0.13 ± 0.01 | 0.47 ± 0.05 | 3.76 | 3.55 ± 0.16 | 6.71 ± 0. 32 | 1.89 | 0.09 ± 0.00 | 0.11 ± 0.01 | 1.15 |
  Summation | 23.88 ± 1.32 | 15.12 ± 0.83 | 0.63 | 1.11 ± 0.06 | 1.54 ± 0.11 | 1.40 | 6.64 ± 0.29 | 7.68 ± 0.36 | 1.16 | 0.31 ± 0.01 | 0.14 ± 0.01 | 0.47 |
Hydroxycinnamic acids derivatives | ||||||||||||
  caffeoylquinic acid | 0.16 ± 0.01 | 0.14 ± 0.01 | 0.90 | 0.14 ± 0.01 | 0.02 ± 0.00 | 0.17 | 0.12 ± 0.01 | 0.06 ± 0.00 | 0.52 | ND | ND |  |
  p-coumaroylquinic acid | 2.29 ± 0.12 | 3.44 ± 0.15 | 1.51 | ND | 0.51 ± 0.02 |  | 0.45 ± 0.04 | 2.11 ± 0.13 | 4.65 | ND | ND |  |
  Summation | 2.45 ± 0.13 | 3.59 ± 0.16 | 1.47 | 0.14 ± 0.01 | 0.53 ± 0.02 | 3.93 | 0.57 ± 0.05 | 2.17 ± 0.13 | 3.78 | ND | ND |  |
Flavanols (mg/g) | ||||||||||||
NongalloylatedCatechins | ||||||||||||
  catechin | 2.79 ± 0.12 | 3.74 ± 0.16 | 1.34 | 0.86 ± 0.04 | 2.59 ± 0.13 | 3.02 | 5.51 ± 0.26 | 1.71 ± 0.08 | 0.31 | 0.99 ± 0.04 | 3.03 ± 0.13 | 3.06 |
  epicatechin | 3.64 ± 0.21 | 6.26 ± 0.29 | 1.72 | 3.37 ± 0.15 | 3.81 ± 0.19 | 1.13 | 8.73 ± 0.31 | 2.75 ± 0.11 | 0.31 | 3.02 ± 0.13 | 4.47 ± 0.15 | 1.48 |
  gallocatechin | 1.00 ± 0.06 | 2.66 ± 0.12 | 2.66 | 1.54 ± 0.08 | 2.30 ± 0.11 | 1.49 | 1.43 ± 0.06 | 1.34 ± 0.07 | 0.93 | 0.24 ± 0.01 | 1.05 ± 0.06 | 4.36 |
  epigallocatechin | 13.91 ± 0.8 | 26.89 ± 1.20 | 1.93 | 10.47 ± 0.62 | 7.93 ± 0.38 | 0.76 | 13.99 ± 0.80 | 12.11 ± 0.71 | 0.87 | 4.24 ± 0.15 | 3.19 ± 0.14 | 0.75 |
  Summation | 21.34 ± 1.19 | 39.55 ± 1.77 | 1.85 | 16.23 ± 0.89 | 16.63 ± 0.71 | 1.02 | 29.67 ± 1.36 | 17.90 ± 0.97 | 0.60 | 8.49 ± 0.33 | 11.74 ± 0.48 | 1.38 |
Galloylatedcatechins | ||||||||||||
  epicatechingallate | 22.38 ± 1.09 | 20.75 ± 1.01 | 0.93 | 3.82 ± 0.15 | 3.58 ± 0.15 | 0.94 | 11.08 ± 0.84 | 9.29 ± 0.83 | 0.84 | 2.18 ± 0.13 | 2.05 ± 0.98 | 0.94 |
  epigallocatechingallate | 89.03 ± 4.21 | 95.88 ± 4.67 | 1.08 | 18.29 ± 0.95 | 14.26 ± 0.68 | 0.78 | 52.19 ± 2.65 | 42.19 ± 2.05 | 0.81 | 6.30 ± 0.31 | 6.46 ± 0.31 | 1.03 |
  Summation | 111.40 ± 5.30 | 116.63 ± 5.68 | 1.05 | 22.11 ± 1.10 | 17.84 ± 0.83 | 0.81 | 63.27 ± 3.49 | 51.48 ± 2.88 | 0.81 | 8.47 ± 0.44 | 8.51 ± 1.29 | 1.00 |
  total Catechins | 132.74 ± 6.49 | 156.18 ± 7.45 | 1.18 | 38.34 ± 1.99 | 34.47 ± 1.85 | 0.90 | 92.94 ± 4.85 | 69.38 ± 3.85 | 0.75 | 16.97 ± 0.77 | 20.25 ± 1.77 | 1.19 |
Proanthocyanidins (area) | ||||||||||||
 m/z 865 | ND | ND |  | ND | ND |  | ND | ND |  | 490 ± 36 | 6057 ± 312 | 12.36 |
 m/z 577 PAs B2 | 33,626 ± 1670 | 52,158 ± 2600 | 1.55 | 17,040 ± 850 | 36,819 ± 1830 | 2.16 | 122,564 ± 6115 | 23,153 ± 1160 | 0.19 | 37,345 ± 1876 | 155,893 ± 7805 | 4.17 |
 m/z 729EC-ECG | 17,582 ± 880 | 18,560 ± 930 | 1.06 | 2125 ± 105 | 3947 ± 185 | 1.86 | 15,214 ± 755 | 7597 ± 380 | 0.50 | 2089 ± 117 | 10,067 ± 515 | 4.82 |
 m/z 593EC-EGC or ECDG | 2300 ± 110 | 6507 ± 320 | 2.83 | 5361 ± 260 | 17,280 ± 855 | 3.22 | 16,556 ± 815 | 5454 ± 267 | 0.33 | 3475 ± 184 | 20,418 ± 1015 | 5.88 |
 m/z 761EGC-EGCG | 11,308 ± 565 | 21,097 ± 1050 | 1.87 | 3841 ± 180 | 4698 ± 225 | 1.22 | 6627 ± 325 | 9111 ± 438 | 1.37 | 909 ± 55 | 4932 ± 264 | 5.43 |
 m/z 745 | 3570 ± 178 | 5468 ± 270 | 1.53 | 2062 ± 105 | 3916 ± 185 | 1.90 | 3806 ± 185 | 3133 ± 148 | 0.82 | ND | 3992 ± 196 |  |
 m/z 609(EGC-EGC) | 3809 ± 190 | 11,528 ± 570 | 3.03 | 11,219 ± 550 | 32,040 ± 1505 | 2.86 | 11,924 ± 585 | 5195 ± 246 | 0.44 | 2501 ± 129 | 17,566 ± 868 | 7.02 |
flavonols derivatives (area) | ||||||||||||
tri-hydroxyl in B-ring | ||||||||||||
  myricetin 3-O-galactoside | 3929 ± 203 | 5100 ± 268 | 1.30 | 705 ± 42 | ND |  | ND | 2367 ± 123 |  | ND | 269 ± 12 |  |
  myricetin 3-O- glucoside | 6797 ± 346 | 6940 ± 359 | 1.02 | 1220 ± 58 | 1301 ± 72 | 1.07 | 3577 ± 185 | 3404 ± 164 | 0.95 | ND | 260 ± 10 |  |
  Summation | 10,726 ± 549 | 12,040 ± 627 | 1.12 | 1925 ± 100 | 1301 ± 72 | 0.68 | 3577 ± 185 | 5771 ± 287 | 1.61 | ND | 529 ± 30 |  |
di-hydroxyl in B-ring | ||||||||||||
  quercetin 3-O-galactosylrutinoside | 2539 ± 136 | 2235 ± 126 | 0.88 | 780 ± 48 | 489 ± 34 | 0.63 | 2465 ± 131 | 1025 ± 55 | 0.42 | 806 ± 45 | 684 ± 28 | 0.85 |
  quercetin3-O-glucosylrutinoside | 9680 ± 496 | 8675 ± 456 | 0.90 | 3933 ± 208 | 2379 ± 126 | 0.60 | 5641 ± 291 | 3847 ± 184 | 0.68 | 793 ± 45 | 2704 ± 136 | 3.41 |
  quercetin 3-galactoside | 1404 ± 87 | 1367 ± 78 | 0.97 | 428 ± 30 | ND |  | 1376 ± 62 | 674 ± 31 | 0.49 | 290 ± 18 | 208 ± 12 | 0.72 |
  quercetin 3-O-glucoside | 2465 ± 138 | 1630 ± 89 | 0.66 | 911 ± 42 | 850 ± 45 | 0.93 | 1284 ± 58 | 783 ± 45 | 0.61 | 168 ± 7 | 526 ± 32 | 3.14 |
  Summation | 16,088 ± 857 | 13,907 ± 749 | 0.86 | 6052 ± 328 | 3717 ± 201 | 0.61 | 10,766 ± 542 | 6330 ± 315 | 0.59 | 2056 ± 115 | 4122 ± 208 | 2.00 |
mono -hydroxyl in B-ring | ||||||||||||
  kaempferol-3-O-galactosylrutinoside | 338,752 ± 16,950 | 290,468 ± 14,530 | 0.86 | 61,932 ± 3085 | 39,007 ± 1968 | 0.63 | 137,928 ± 6870 | 130,099 ± 6485 | 0.94 | 23,498 ± 1164 | 18,979 ± 1001 | 0.81 |
  kaempferol3-O-glucosylrutinoside | 853,325 ± 42,664 | 753,945 ± 37,665 | 0.88 | 206,694 ± 10,345 | 120,862 ± 6055 | 0.58 | 316,408 ± 15,808 | 334,177 ± 16,675 | 1.06 | 23,691 ± 1135 | 37,778 ± 1982 | 1.59 |
  kaempferol-3-O-galactoside | ND | 933 ± 59 |  | 154 ± 10 | 287 ± 28 | 1.86 | 447 ± 31 | 484 ± 30 | 1.08 | ND | 255 ± 10 |  |
  kaempferol-3-O-glucoside | ND | 20,072 ± 1008 |  | 1491 ± 85 | ND |  | 6994 ± 350 | 9054 ± 446 | 1.29 | 496 ± 71 | 199 ± 9 | 0.40 |
  Kaempferol-3-O-rhamnosylgalactoside | 25,559 ± 1289 | 26,315 ± 1315 | 1.03 | 11,173 ± 560 | 5296 ± 276 | 0.47 | 9333 ± 456 | 11,450 ± 564 | 1.23 | 1567 ± 76 | 2223 ± 124 | 1.42 |
  Summation | 1,217,636 ± 60,903 | 1,091,733 ± 54,577 | 0.90 | 281,445 ± 14,085 | 165,452 ± 8327 | 0.59 | 471,109 ± 23,515 | 485,263 ± 24,200 | 1.03 | 49,252 ± 2396 | 59,434 ± 3126 | 1.21 |
 total flavonols | 1,244,449 ± 62,309 | 1,117,680 ± 55,953 | 0.90 | 289,422 ± 14,513 | 170,470 ± 8600 | 0.59 | 485,453 ± 24,242 | 497,364 ± 24,802 | 1.02 | 51,309 ± 2511 | 63,556 ± 3334 | 1.24 |