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Table 1 Effects of sucrose on polyphenol accumulation in different tissues of tea plantlets after 14d treatment using UPLC-QQQ-MS/MS

From: Effects of vitro sucrose on quality components of tea plants (Camellia sinensis) based on transcriptomic and metabolic analysis

Compound Control Suc ratio Control Suc ratio Control Suc ratio Control Suc ratio
bud bud   3rd leaf 3rd leaf   up-stem up-stem down-stem down-stem
Phenolic acid (mg/g)
 Quinic acid 44.21 ± 2.01 86.06 ± 4.05 1.95 6.55 ± 0.23 7.45 ± 0.35 1.14 39.43 ± 1.89 40.19 ± 1.70 1.02 3.72 ± 0.15 6.19 ± 0.29 1.67
Gallic acid derivatives
  β-glucogallin 9.42 ± 0.41 1.47 ± 0.11 0.16 0.90 ± 0.05 0.97 ± 0.05 1.08 2.83 ± 0.12 0.81 ± 0.03 0.29 0.02 ± 0.00 0.01 ± 0.00 0.73
  galloyl acid 0.38 ± 0.01 0.36 ± 0.02 0.95 0.08 ± 0.00 0.10 ± 0.01 1.19 0.25 ± 0.01 0.16 ± 0.01 0.65 0.20 ± 0.01 0.03 ± 0.00 0.13
  galloylquinic acid 14.09 ± 0.9 13.29 ± 0.7 0.94 0.13 ± 0.01 0.47 ± 0.05 3.76 3.55 ± 0.16 6.71 ± 0. 32 1.89 0.09 ± 0.00 0.11 ± 0.01 1.15
  Summation 23.88 ± 1.32 15.12 ± 0.83 0.63 1.11 ± 0.06 1.54 ± 0.11 1.40 6.64 ± 0.29 7.68 ± 0.36 1.16 0.31 ± 0.01 0.14 ± 0.01 0.47
Hydroxycinnamic acids derivatives
  caffeoylquinic acid 0.16 ± 0.01 0.14 ± 0.01 0.90 0.14 ± 0.01 0.02 ± 0.00 0.17 0.12 ± 0.01 0.06 ± 0.00 0.52 ND ND  
  p-coumaroylquinic acid 2.29 ± 0.12 3.44 ± 0.15 1.51 ND 0.51 ± 0.02   0.45 ± 0.04 2.11 ± 0.13 4.65 ND ND  
  Summation 2.45 ± 0.13 3.59 ± 0.16 1.47 0.14 ± 0.01 0.53 ± 0.02 3.93 0.57 ± 0.05 2.17 ± 0.13 3.78 ND ND  
Flavanols (mg/g)
NongalloylatedCatechins
  catechin 2.79 ± 0.12 3.74 ± 0.16 1.34 0.86 ± 0.04 2.59 ± 0.13 3.02 5.51 ± 0.26 1.71 ± 0.08 0.31 0.99 ± 0.04 3.03 ± 0.13 3.06
  epicatechin 3.64 ± 0.21 6.26 ± 0.29 1.72 3.37 ± 0.15 3.81 ± 0.19 1.13 8.73 ± 0.31 2.75 ± 0.11 0.31 3.02 ± 0.13 4.47 ± 0.15 1.48
  gallocatechin 1.00 ± 0.06 2.66 ± 0.12 2.66 1.54 ± 0.08 2.30 ± 0.11 1.49 1.43 ± 0.06 1.34 ± 0.07 0.93 0.24 ± 0.01 1.05 ± 0.06 4.36
  epigallocatechin 13.91 ± 0.8 26.89 ± 1.20 1.93 10.47 ± 0.62 7.93 ± 0.38 0.76 13.99 ± 0.80 12.11 ± 0.71 0.87 4.24 ± 0.15 3.19 ± 0.14 0.75
  Summation 21.34 ± 1.19 39.55 ± 1.77 1.85 16.23 ± 0.89 16.63 ± 0.71 1.02 29.67 ± 1.36 17.90 ± 0.97 0.60 8.49 ± 0.33 11.74 ± 0.48 1.38
Galloylatedcatechins
  epicatechingallate 22.38 ± 1.09 20.75 ± 1.01 0.93 3.82 ± 0.15 3.58 ± 0.15 0.94 11.08 ± 0.84 9.29 ± 0.83 0.84 2.18 ± 0.13 2.05 ± 0.98 0.94
  epigallocatechingallate 89.03 ± 4.21 95.88 ± 4.67 1.08 18.29 ± 0.95 14.26 ± 0.68 0.78 52.19 ± 2.65 42.19 ± 2.05 0.81 6.30 ± 0.31 6.46 ± 0.31 1.03
  Summation 111.40 ± 5.30 116.63 ± 5.68 1.05 22.11 ± 1.10 17.84 ± 0.83 0.81 63.27 ± 3.49 51.48 ± 2.88 0.81 8.47 ± 0.44 8.51 ± 1.29 1.00
  total Catechins 132.74 ± 6.49 156.18 ± 7.45 1.18 38.34 ± 1.99 34.47 ± 1.85 0.90 92.94 ± 4.85 69.38 ± 3.85 0.75 16.97 ± 0.77 20.25 ± 1.77 1.19
Proanthocyanidins (area)
 m/z 865 ND ND   ND ND   ND ND   490 ± 36 6057 ± 312 12.36
 m/z 577 PAs B2 33,626 ± 1670 52,158 ± 2600 1.55 17,040 ± 850 36,819 ± 1830 2.16 122,564 ± 6115 23,153 ± 1160 0.19 37,345 ± 1876 155,893 ± 7805 4.17
 m/z 729EC-ECG 17,582 ± 880 18,560 ± 930 1.06 2125 ± 105 3947 ± 185 1.86 15,214 ± 755 7597 ± 380 0.50 2089 ± 117 10,067 ± 515 4.82
 m/z 593EC-EGC or ECDG 2300 ± 110 6507 ± 320 2.83 5361 ± 260 17,280 ± 855 3.22 16,556 ± 815 5454 ± 267 0.33 3475 ± 184 20,418 ± 1015 5.88
 m/z 761EGC-EGCG 11,308 ± 565 21,097 ± 1050 1.87 3841 ± 180 4698 ± 225 1.22 6627 ± 325 9111 ± 438 1.37 909 ± 55 4932 ± 264 5.43
 m/z 745 3570 ± 178 5468 ± 270 1.53 2062 ± 105 3916 ± 185 1.90 3806 ± 185 3133 ± 148 0.82 ND 3992 ± 196  
 m/z 609(EGC-EGC) 3809 ± 190 11,528 ± 570 3.03 11,219 ± 550 32,040 ± 1505 2.86 11,924 ± 585 5195 ± 246 0.44 2501 ± 129 17,566 ± 868 7.02
flavonols derivatives (area)
tri-hydroxyl in B-ring
  myricetin 3-O-galactoside 3929 ± 203 5100 ± 268 1.30 705 ± 42 ND   ND 2367 ± 123   ND 269 ± 12  
  myricetin 3-O- glucoside 6797 ± 346 6940 ± 359 1.02 1220 ± 58 1301 ± 72 1.07 3577 ± 185 3404 ± 164 0.95 ND 260 ± 10  
  Summation 10,726 ± 549 12,040 ± 627 1.12 1925 ± 100 1301 ± 72 0.68 3577 ± 185 5771 ± 287 1.61 ND 529 ± 30  
di-hydroxyl in B-ring
  quercetin 3-O-galactosylrutinoside 2539 ± 136 2235 ± 126 0.88 780 ± 48 489 ± 34 0.63 2465 ± 131 1025 ± 55 0.42 806 ± 45 684 ± 28 0.85
  quercetin3-O-glucosylrutinoside 9680 ± 496 8675 ± 456 0.90 3933 ± 208 2379 ± 126 0.60 5641 ± 291 3847 ± 184 0.68 793 ± 45 2704 ± 136 3.41
  quercetin 3-galactoside 1404 ± 87 1367 ± 78 0.97 428 ± 30 ND   1376 ± 62 674 ± 31 0.49 290 ± 18 208 ± 12 0.72
  quercetin 3-O-glucoside 2465 ± 138 1630 ± 89 0.66 911 ± 42 850 ± 45 0.93 1284 ± 58 783 ± 45 0.61 168 ± 7 526 ± 32 3.14
  Summation 16,088 ± 857 13,907 ± 749 0.86 6052 ± 328 3717 ± 201 0.61 10,766 ± 542 6330 ± 315 0.59 2056 ± 115 4122 ± 208 2.00
mono -hydroxyl in B-ring
  kaempferol-3-O-galactosylrutinoside 338,752 ± 16,950 290,468 ± 14,530 0.86 61,932 ± 3085 39,007 ± 1968 0.63 137,928 ± 6870 130,099 ± 6485 0.94 23,498 ± 1164 18,979 ± 1001 0.81
  kaempferol3-O-glucosylrutinoside 853,325 ± 42,664 753,945 ± 37,665 0.88 206,694 ± 10,345 120,862 ± 6055 0.58 316,408 ± 15,808 334,177 ± 16,675 1.06 23,691 ± 1135 37,778 ± 1982 1.59
  kaempferol-3-O-galactoside ND 933 ± 59   154 ± 10 287 ± 28 1.86 447 ± 31 484 ± 30 1.08 ND 255 ± 10  
  kaempferol-3-O-glucoside ND 20,072 ± 1008   1491 ± 85 ND   6994 ± 350 9054 ± 446 1.29 496 ± 71 199 ± 9 0.40
  Kaempferol-3-O-rhamnosylgalactoside 25,559 ± 1289 26,315 ± 1315 1.03 11,173 ± 560 5296 ± 276 0.47 9333 ± 456 11,450 ± 564 1.23 1567 ± 76 2223 ± 124 1.42
  Summation 1,217,636 ± 60,903 1,091,733 ± 54,577 0.90 281,445 ± 14,085 165,452 ± 8327 0.59 471,109 ± 23,515 485,263 ± 24,200 1.03 49,252 ± 2396 59,434 ± 3126 1.21
 total flavonols 1,244,449 ± 62,309 1,117,680 ± 55,953 0.90 289,422 ± 14,513 170,470 ± 8600 0.59 485,453 ± 24,242 497,364 ± 24,802 1.02 51,309 ± 2511 63,556 ± 3334 1.24
  1. Note: ND indicates that the polyphenol was not detected; the data represents the mean value of three biological replicates
  2. Digit indicates the ratio of Suc / Control