Fig. 6

Validation of lncRNAs with virus-induced gene silencing. a Relative expression levels of S-slylnc0957 using real-time RT-PCR analysis in the VIGS-treated tomato plants 20 days after agroinfiltration with TRV2 vectors. Tomato α-tubulin (Solyc04g077020.2) was used as an internal reference. Error bars represented standard errors of three biological replicates, and asterisks indicate significant differences based on the Student’s t test (P < 0.05). b TYLCV accumulation in the S-slylnc0957 silenced plants was estimated from the total genomic DNA by quantitative RT-PCR. Values were normalized using tomato α-tubulin (Solyc04g077020.2) as an internal reference. Error bars represented standard errors of three biological replicates and asterisk indicates significant difference based on the Student’s t test (P < 0.05). c Cotyledon agroinfiltration of TRV vectors with TYLCV infection was performed in the susceptible tomato at the cotyledon stage. Plants treated with the pTRV vectors showed the susceptible phenotype (Left). Susceptible plantlets treated with the S-slylnc0957 gene silencing constructs pTRV1 and pTRV2-S-slylnc0957 have no symptoms (right)