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Table 1 Summary of T0 transgenic wheat plants regenerated in 19 independent events of biolistic transformations

From: A biolistic method for high-throughput production of transgenic wheat plants with single gene insertions

Experiment

No. trans-formed embryos

Transgene copy No.

No. hygromycin resistant plants (HmR)

No. GOI transformants

GOI with one copy transformants (%)

Transformation frequency, TF, HmR (%)

Co-transformation, Co-TF (%)

0

1

2

≥3

T01

857

18

19

4

17

58

40

47.5

6.8

69.0

T02

998

36

29

7

40

112

76

38.2

11.2

67.9

T03

1005

21

22

5

29

77

56

39.3

7.7

72.7

T04

912

21

20

2

56

99

78

25.6

10.8

78.8

T05

899

14

23

7

44

88

74

31.1

9.8

84.1

T06

753

18

15

0

13

46

28

53.6

6.1

60.9

T07

950

37

60

31

65

193

156

38.5

20.3

80.8

T08

914

62

48

14

28

152

90

53.3

16.6

59.2

T09

936

38

51

16

6

111

73

69.9

11.9

65.8

T10

735

10

25

14

38

87

77

32.5

11.8

88.5

T11

729

20

19

7

82

128

108

17.6

17.6

84.4

T12

662

14

25

8

1

48

34

73.5

7.2

70.8

T13

620

2

15

6

72

95

93

16.1

15.3

97.9

T14

708

11

30

2

38

81

70

42.9

11.4

84.6

T15

847

33

6

2

7

48

15

40.0

5.7

31.3

T16

856

16

8

5

7

36

20

40.0

4.2

55.6

T17

777

5

8

4

7

24

19

42.1

3.1

79.2

T18

681

1

7

4

10

22

21

33.3

3.2

94.5

T19

657

12

9

1

11

33

21

42.9

5.0

63.6

Total

15,496

389

439

139

571

1538

1149

   

Transformation, %

100

    

9.9

7.4

  

74.7

GOI transformation, %

 

38.2

12.1

49.7

 

100

   
  1. Data summarized for 19 stable transformation experiments. The transgene copy numbers of GOI in each event were determined by qPCR and confirmed by the Southern blot