Experiment | No. trans-formed embryos | Transgene copy No. | No. hygromycin resistant plants (HmR) | No. GOI transformants | GOI with one copy transformants (%) | Transformation frequency, TF, HmR (%) | Co-transformation, Co-TF (%) | |||
---|---|---|---|---|---|---|---|---|---|---|
0 | 1 | 2 | ≥3 | |||||||
T01 | 857 | 18 | 19 | 4 | 17 | 58 | 40 | 47.5 | 6.8 | 69.0 |
T02 | 998 | 36 | 29 | 7 | 40 | 112 | 76 | 38.2 | 11.2 | 67.9 |
T03 | 1005 | 21 | 22 | 5 | 29 | 77 | 56 | 39.3 | 7.7 | 72.7 |
T04 | 912 | 21 | 20 | 2 | 56 | 99 | 78 | 25.6 | 10.8 | 78.8 |
T05 | 899 | 14 | 23 | 7 | 44 | 88 | 74 | 31.1 | 9.8 | 84.1 |
T06 | 753 | 18 | 15 | 0 | 13 | 46 | 28 | 53.6 | 6.1 | 60.9 |
T07 | 950 | 37 | 60 | 31 | 65 | 193 | 156 | 38.5 | 20.3 | 80.8 |
T08 | 914 | 62 | 48 | 14 | 28 | 152 | 90 | 53.3 | 16.6 | 59.2 |
T09 | 936 | 38 | 51 | 16 | 6 | 111 | 73 | 69.9 | 11.9 | 65.8 |
T10 | 735 | 10 | 25 | 14 | 38 | 87 | 77 | 32.5 | 11.8 | 88.5 |
T11 | 729 | 20 | 19 | 7 | 82 | 128 | 108 | 17.6 | 17.6 | 84.4 |
T12 | 662 | 14 | 25 | 8 | 1 | 48 | 34 | 73.5 | 7.2 | 70.8 |
T13 | 620 | 2 | 15 | 6 | 72 | 95 | 93 | 16.1 | 15.3 | 97.9 |
T14 | 708 | 11 | 30 | 2 | 38 | 81 | 70 | 42.9 | 11.4 | 84.6 |
T15 | 847 | 33 | 6 | 2 | 7 | 48 | 15 | 40.0 | 5.7 | 31.3 |
T16 | 856 | 16 | 8 | 5 | 7 | 36 | 20 | 40.0 | 4.2 | 55.6 |
T17 | 777 | 5 | 8 | 4 | 7 | 24 | 19 | 42.1 | 3.1 | 79.2 |
T18 | 681 | 1 | 7 | 4 | 10 | 22 | 21 | 33.3 | 3.2 | 94.5 |
T19 | 657 | 12 | 9 | 1 | 11 | 33 | 21 | 42.9 | 5.0 | 63.6 |
Total | 15,496 | 389 | 439 | 139 | 571 | 1538 | 1149 | Â | Â | Â |
Transformation, % | 100 | Â | Â | Â | Â | 9.9 | 7.4 | Â | Â | 74.7 |
GOI transformation, % |  | – | 38.2 | 12.1 | 49.7 |  | 100 |  |  |  |