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Table 1 Summary of T0 transgenic wheat plants regenerated in 19 independent events of biolistic transformations

From: A biolistic method for high-throughput production of transgenic wheat plants with single gene insertions

Experiment No. trans-formed embryos Transgene copy No. No. hygromycin resistant plants (HmR) No. GOI transformants GOI with one copy transformants (%) Transformation frequency, TF, HmR (%) Co-transformation, Co-TF (%)
0 1 2 ≥3
T01 857 18 19 4 17 58 40 47.5 6.8 69.0
T02 998 36 29 7 40 112 76 38.2 11.2 67.9
T03 1005 21 22 5 29 77 56 39.3 7.7 72.7
T04 912 21 20 2 56 99 78 25.6 10.8 78.8
T05 899 14 23 7 44 88 74 31.1 9.8 84.1
T06 753 18 15 0 13 46 28 53.6 6.1 60.9
T07 950 37 60 31 65 193 156 38.5 20.3 80.8
T08 914 62 48 14 28 152 90 53.3 16.6 59.2
T09 936 38 51 16 6 111 73 69.9 11.9 65.8
T10 735 10 25 14 38 87 77 32.5 11.8 88.5
T11 729 20 19 7 82 128 108 17.6 17.6 84.4
T12 662 14 25 8 1 48 34 73.5 7.2 70.8
T13 620 2 15 6 72 95 93 16.1 15.3 97.9
T14 708 11 30 2 38 81 70 42.9 11.4 84.6
T15 847 33 6 2 7 48 15 40.0 5.7 31.3
T16 856 16 8 5 7 36 20 40.0 4.2 55.6
T17 777 5 8 4 7 24 19 42.1 3.1 79.2
T18 681 1 7 4 10 22 21 33.3 3.2 94.5
T19 657 12 9 1 11 33 21 42.9 5.0 63.6
Total 15,496 389 439 139 571 1538 1149    
Transformation, % 100      9.9 7.4    74.7
GOI transformation, %   38.2 12.1 49.7   100    
  1. Data summarized for 19 stable transformation experiments. The transgene copy numbers of GOI in each event were determined by qPCR and confirmed by the Southern blot