Fig. 4

Example of Southern blot of transgenic wheat plants with different copy numbers of the transgene. Four out of the eight transgenic lines had single transgene insertions (underlined). Four other transgenic lines (not underlined) had multiple copy numbers of the transgene. A positive control, consisting of DNA from a barley plant with a single transgene insertion is indicated as the ‘Control’. The negative control is represented by wild-type (WT) wheat DNA (cv. Gladius)