Fig. 5

Localization of the chromosome 9-specific oligo library, 45S rDNA and CentO probes on the chromosomes of O. eichingeri and a triploid (AAC). The chromosome 9-specific (red) and 45S rDNA probes (green) fluorescing in prometaphase chromosomes of the (a) O. eichingeri (CC, 2n = 2× = 24) and (c) the triploid (AAC, 2n = 3× = 36) derived from O. eichingeri and 02428. b and d The chromosome 9-specific and 45S rDNA signals were digitally separated from the rest of the chromosomes in panels (a) and (c), respectively. e The chromosome 9-specific (red) and CentO (green) probes on chromosomes of the triploid. f The chromosome 9-specific and CentO signals were digitally separated from the rest of the chromosomes in (e). White arrows indicate the chromosome 9 from O. eichingeri. Chromosomes were stained with DAPI. Bars, 5 μm