Fig. 2From: Role of auxin (IAA) in the regulation of slow vacuolar (SV) channels and the volume of red beet taproot vacuolesEffect of cytosolic IAA on the slow vacuolar (SV) channels in red beet taproot vacuoles. a An example of an SV current recording for a single vacuole in the control bath (control at 0 time, recorded immediately after the establishment of the whole-vacuole configuration as well as 5 min later) and in the presence of IAA at 1 μM (auxin was added to the bath immediately after the current was recorded in the control at 0 time; however, the current in the presence of IAA was recorded 5 min after the control at 0 time). SV currents elicited by a series of voltage steps ranging from − 100 to + 100 mV in 10 mV steps; holding potential 0 mV. b Steady-state currents (normalized to the current amplitude at + 100 mV under control at 0 min) were determined in the control medium (control at 0 and 5 min) and in the presence of 0.1, 1 and 10 μM IAA. The current traces were fitted with the exponential function: i(t) = a + b (1-exp(−t/τ)), where a - current at t = 0, b - current at saturation (plateau), t - time and τ - time constant. The steady state is the difference between current at saturation (plateau) and current at time “0” (leak). Data points are the means (± SE) from at least seven experiments performed with different vacuoles. The significance of the results was analyzed for voltage + 100 mV using the post hoc least significant difference (LSD) test. Means followed by the same letter are not significantly different from each other (LSD test P < 0.05)Back to article page