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Fig. 2 | BMC Plant Biology

Fig. 2

From: Role of auxin (IAA) in the regulation of slow vacuolar (SV) channels and the volume of red beet taproot vacuoles

Fig. 2

Effect of cytosolic IAA on the slow vacuolar (SV) channels in red beet taproot vacuoles. a An example of an SV current recording for a single vacuole in the control bath (control at 0 time, recorded immediately after the establishment of the whole-vacuole configuration as well as 5 min later) and in the presence of IAA at 1 μM (auxin was added to the bath immediately after the current was recorded in the control at 0 time; however, the current in the presence of IAA was recorded 5 min after the control at 0 time). SV currents elicited by a series of voltage steps ranging from − 100 to + 100 mV in 10 mV steps; holding potential 0 mV. b Steady-state currents (normalized to the current amplitude at + 100 mV under control at 0 min) were determined in the control medium (control at 0 and 5 min) and in the presence of 0.1, 1 and 10 μM IAA. The current traces were fitted with the exponential function: i(t) = a + b (1-exp(−t/τ)), where a - current at t = 0, b - current at saturation (plateau), t - time and τ - time constant. The steady state is the difference between current at saturation (plateau) and current at time “0” (leak). Data points are the means (± SE) from at least seven experiments performed with different vacuoles. The significance of the results was analyzed for voltage + 100 mV using the post hoc least significant difference (LSD) test. Means followed by the same letter are not significantly different from each other (LSD test P < 0.05)

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