Fig. 7

Cytosine methylation levels in the 5′ upstream region of the MdMYB1 gene in apple skin after 5-aza-2′-deoxycytidine (5-aza-2’-dC) treatment. Cytosine methylation levels of the MdMYB1 promoter estimated in ‘Granny Smith’ (a) and ‘Golden Delicious’ (b). c: Methylation levels of the three cytosine contexts in the MdMYB1 promoter in ‘Granny Smith’ and ‘Golden Delicious’. Fruit skins were harvested at 0, 9, and 15 d after 5-aza-dC treatment. The product of bisulfite modification was used as a template to amplify the MdMYB1 promoter region using eleven primer pairs, and twenty independent clones from each PCR were sequenced and analyzed by Kismeth. The X axis in A and B indicates the nucleotide positions relative to the ATG translation start site. H indicates A, C, or T. Asterisks indicate significantly different values (*p < 0.05; **p < 0.01)