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Fig. 4 | BMC Plant Biology

Fig. 4

From: The effect of promoter methylation on MdMYB1 expression determines the level of anthocyanin accumulation in skins of two non-red apple cultivars

Fig. 4

Cytosine methylation levels in the 5′ upstream region of the MdMYB1 gene in fruit skin after bag removal. Cytosine methylation levels in the MdMYB1 promoter estimated in ‘Granny Smith’ (a) and ‘Golden Delicious’ (b). c: Methylation levels for the different cytosine contexts in the MdMYB1 promoter in ‘Granny Smith’ and ‘Golden Delicious’. Fruit skins were harvested at 0, 4, and 10 d after bag removal treatment. The product of bisulfite modification was used as a template to amplify the MdMYB1 promoter region using eleven primer pairs, and twenty independent clones from each PCR were sequenced and analyzed using the Kismeth software. The X axis in A and B indicates the nucleotide positions relative to the ATG translation start site. H indicates A, C, or T. Asterisks indicate significantly different values (* p < 0.05; ** p < 0.01)

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