Fig. 6
From: Molecular and biochemical characterization of a novel isoprene synthase from Metrosideros polymorpha
Isoprene production in E. coli. a Schematic representation of isoprene production pathway in an engineered E. coli harboring mevalonate (MVA) pathway genes. The isoprene production pathway from acetyl-CoA consists of seven enzymes: MvaE, Acetyl-CoA acetyltransferase/HMG-CoA reductase; MvaS, 3-hydroxy-3-methylglutaryl-CoA synthase; MvaK1, mevalonate kinase; MvaK2, phosphomevalonate kinase; MvaD, mevalonate 5-diphosphate decarboxylase; Idi, isopentenyl diphosphate isomerase; and MpIspS, isoprene synthase. All enzymes were expressed from IPTG-inducible promoters (Ptrc promoter for isoprene synthase, Plac promoter for mevalonate pathway). b Isoprene production at 30 °C by E. coli expressing MpIspS and MVA pathway genes with or without IPTG. Isoprene in the headspace was measured after 72 h incubation at 30 °C