Fig. 5From: Molecular and biochemical characterization of a novel isoprene synthase from Metrosideros polymorphaMichaelis–Menten kinetics Michaelis–Menten plot of MpIspS initial velocity versus substrate (DMAPP) concentration, monitoring production of isoprene. Reaction was in 50 mM MOPS buffer (pH 6.0) containing 5 mM Mn2+ and indicated concentration of DMAPP at 50 °C for 10 minBack to article page