Fig. 2
From: Molecular and biochemical characterization of a novel isoprene synthase from Metrosideros polymorpha
SDS-PAGE analysis and determination of the molecular mass of MpIspS. a Map of MpIspS. Essential components of the vector are indicated. The MpIspS coding region was cloned between the T7 promoter and terminator in vector pET-28a(+) containing the N-terminal His6 tag. b SDS-PAGE of MpIspS. Lanes: 1, pre-stained marker protein (250, 150, 100, 75, 50, 37, and 25 kDa); 2, crude extract; 3, purified enzyme. c. Determination of the molecular mass of MpIspS by gel-filtration chromatography. Reference proteins as closed circle were apoferritin (443 kDa), β-amylase (200 kDa), alcohol dehydrogenase (150 kDa), albumin (13.7 kDa), and carbonic anhydrase (29 kDa). MpIspS is represented by the red triangle