Mapping QTLs for water-use efficiency reveals the potential candidate genes involved in regulating the trait in apple under drought stress

Table 1 Quality evaluation of RAD-seq data for parents and progeny

Sample		Clean bases (Gb)	Clean reads (M)	Q20 (%)	Q30 (%)	GC content (%)	Coverage of genome^a(X)	Depth of RAD tags^b (X)
Parents	Honeycrisp	23.31	76.95	95.38	89.75	37.42	31.41	77.75
	Qinguan	20.15	66.93	93.73	86.91	38.47	27.16	72.24
Progeny	Average	1.05	3.50	94.08	87.30	37.64
	Maximum	3.02	10.07	96.53	91.75	40.14	4.07	23.88
	Minimum	0.52	1.75	92.55	85.00	35.78	0.71	8.76
	Total	357.15	1191.18
All samples		400.61	1335.06

^a Coverage of genome meant the proportion of total clean bases aligned to apple reference genome accounted for the genome size. The size of apple reference genome was 742.3 Mb estimated by Velasco et al.[35]. ^bDepth of RAD tags meant the sequencing depth of filtered RAD tags

ISSN: 1471-2229