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Fig. 2 | BMC Plant Biology

Fig. 2

From: Functional analysis of a pathogenesis-related thaumatin-like protein gene TaLr35PR5 from wheat induced by leaf rust fungus

Fig. 2

Yeast signal sequence trap assay of the predicted signal peptide of TaLr35PR5 protein. The predicted signal peptide sequences plus the following two amino acids (1–23) of TaLr35PR5, the positive control pSUC2:: Ps87 and negative control pSUC2::Mg87 were used for the assay. CMD-W (minus Trp) plates were used to select yeast strain YTK12 carrying the pSUC2 vector. Sucrose and YPRAA media were used to indicate invertase secretion. An enzymatic activity test based on conversion of the dye 2, 3, 5-triphenyltetrazolium chloride (TTC) to the insoluble red colored triphenylformazan was used to confirm invertase secretion

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