Fig. 5

Metabolite classification and KEGG pathway analysis. a Classification of the significantly varying metabolites between the two developmental stages. The 49 varying metabolites involved amino acids (10), organic acids (12), carbohydrates (4), purines (3), lipids (2), pyrimidines (2), and others (14). b Signalling pathways of the differential metabolites involved in the budding to mid-flowering stages of alfalfa development