Table 2 Sequences of marker primers used to distinguish between green and purple cabbages
From: Purple Brassica oleracea var. capitata F. rubra is due to the loss of BoMYBL2–1 expression
Use | Forward primer | Reverse primer | ||
|---|---|---|---|---|
Name | Sequence | Name | Sequence | |
Wild-type BoMYBL2–1 (947 bp) Variant BoMYBL2–1 (1148 bp) Substituted BoMYBL2–1 (2075 bp) Actin 2 (144 bp) | BoMYBL2–1w-F | 5’-CTACCAGTCTCTCCTTTGAAGAAGAC | BoMYBL2–1w-R | 5’-GAGTTTTCCTTGATCTCACAGTACATTTCT |
BoMYBL2–1v-F | 5’-TGTCCACTATCAACAAAAGATGATC | BoMYBL2–1v-R | 5’-CAAACTCACCCGTGCAAATGTACAT | |
BoMYBL2–1sub-F | 5’-GTTCAAGCTCATTGTGAACGGA | BoMYBL2–1sub-R | 5’-GGACCACCGTGAGAGAGAGA | |
BoACT2-F | 5’-TACGGTAACATCGTGCTCAGTG | BoACT2-R | 5’-GATCCAGACACTGTACTTCCTC | |