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Fig. 7 | BMC Plant Biology

Fig. 7

From: Long non-coding RNAs of switchgrass (Panicum virgatum L.) in multiple dehydration stresses

Fig. 7

Verification of ABA metabolism and dehydration memory lncRNAs using quantitative real-time PCR and HPLC-MS. a, b Relative expression of lncRNAs involved in ABA biosynthesis. c Relative expression of ortholog gene of NCED. d ABA contents in multiple dehydration stresses determined by HPLC-MS. e, f Relative expression of ABA-responsive element binding factors (AREBs/ABFs). g Relative expression of upregulated dehydration memory lncRNA. h Relative expression of downregulated dehydration memory lncRNA. c, control; D1–2, first and second dehydration stresses; R1, first recovery period. Relative gene expressed levels were calculated using the ΔΔCT method with PveEF-1α as the internal control, and three biological replicates were performed for each experiment

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