| | RH hotspot | | hQTL | | Traitse |
|---|
Populationa | Location (Mb) | RHR (%)b | | Interval (Mb)c | LOD | R2 (%) | Additived | QTL type | RH hotspotf | hQTL |
|---|
Hot1 | DE3/BY815 | Chr5:81–89 | 10.6/3.7 | hQTL1 |
Chr5:68.8–92.4
| 7.2 | 14.5 | 0.182♀ | cis | – | – |
hQTL2 | Chr5:151.7–159.3 | 14.3 | 28.2 | 0.213♀ | trans | – | – |
Hot2 | K22/BY815 | Chr5:182–197 | 11.3/3.4 | hQTL3 | Chr5:204.2–208.1 | 8.2 | 17.7 | 0.122♀ | trans | TBN, HKW | TBN |
Hot3 | K22/BY815 | Chr5:204–211 | 11.0/3.4 | hQTL4 |
Chr5:208.1–208.9
| 13.3 | 19.0 | 0.094♀ | cis | ULA, HKW, KW, KT | KW |
Hot4 | K22/CI7 | Chr4:174–181 | 8.0/2.8 | hQTL5 | Chr5:22.1–29.3 | 4.4 | 9.2 | 0.080♂ | trans |
LNAE
| – |
Hot5 | KUI3/SC55 | Chr1:246–251 | 9.4/3.5 | hQTL6 |
Chr1:243.8–247.2
| 4.3 | 8.3 | 0.072♂ | cis | na | na |
Hot6 | YU87–1/BK | Chr1:221–227 | 9.3/3.6 | hQTL7 | Chr1:260.9–269.2 | 6.1 | 15.6 | 0.134♂ | trans |
LW
| – |
Hot7 | ZHENG58/SK | Chr6:136–144 | 8.2/2.9 | hQTL8 | Chr3:224.0–224.5 | 3.4 | 6.4 | 0.062♀ | trans | KNPR, EL, EW | – |
- aPopulation in which the specific RH hotspot was identified; the name of the population is the names of the female and male parents separated by a backslash
- bAverage residual heterozygosity rate within the hotspot region (before backslash) and at the genomic level (after backslash) for each population
- cChromosome and physical location of the hQTL for each hotspot
- dAdditive effect of each hQTL; a value marked with “♀” indicates that the allele from the female parent increases RHR, while a value marked with “♂” indicates that the allele from the male parent increases RHR in the hotspot
- eThe trait represents the collected phenotypes that exhibited significant differences either between heterozygotes and homozygotes within RH hotspots or between two homozygous genotypes for hQTLs (P < 0.05). “-” indicates that no significant trait existed for this hotspot; and “na” indicates that no phenotypic data were available in this population. TBN: tassel branch number. HKW: one hundred kernel weight. ULA: upper ear leaf angle. KW: kernel width. KT: kernel thickness. LNAE: leaf number above ear. LW: leaf width. KNPR: kernel number per row of ear. EL: ear length. EW: ear weight
- fFor underlined traits, the heterozygous genotypes show higher phenotype values than the recessive homozygous genotypes (P < 0.05) but present no difference from the dominant homozygous genotypes, while for traits that are not underlined, the heterozygous genotypes contribute significantly to higher phenotype values than the genotypes of both homozygotes (P < 0.05). In this analysis, the dominant and recessive homozygotes were defined as the homozygotes responsible for the higher and lower phenotype values per trait, respectively
