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Fig. 6 | BMC Plant Biology

Fig. 6

From: Biochemical and molecular characterisation of exogenous cytokinin application on grain filling in rice

Fig. 6

Flowcytometric analysis of ploidy (DNA class, 3C or > 3C) status of the endosperm nuclei in the apical and basal spikelets sampled on 12 DAA from control and 50 μM BAP treated rice cultivar OR-1918 (a), and quantification of the ploidy status of nuclei (b). The endosperm cells of the caryopses released in solution by cellulase treatment were stained with 4′,6-diamidino-2-phenylindole (DAPI) for counting the cells (nuclei) representing various ploidy levels (3C and > 3C). Each panel is representative of three counts from independent endosperm cell preparations. The vertical bars represent mean ± SD of ploidy status of the nuclei in three independent endosperm cell preparations. The bar pair marked by ‘**’ and ‘***’ indicate that the percentage of nuclei representing a ploidy status (3C or > 3C) in the endosperm cells from control and BAP treated plants differed significantly at p ≤ 0.01 and p ≤ 0.001, respectively, as revealed by ‘t’ test (b)

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