Fig. 3

RT-qPCR results representing relative levels of expression of cytokinin oxidase (CKX) isoforms in the spikelets sampled on various days after anthesis (DAA) from rice cultivars grown in field (a) and in pots (b): a) relative expression in apical spikelets over basal spikelets; b) relative expression in the apical and basal spikelets of the BAP treated plants over that of the control plants. Actin served as the reference control. Other experimental details as in Fig. 2. Plus (+)/minus (−) signs against the vertical bars representing individual genes indicate significantly greater/lesser relative expression of the gene in the apical spikelets compared with the basal ones (a), or in spikelelets sampled from the treated plants compared with that sampled from the control plants (b) at least at p ≤ 0.05, as determined by ‘t’ test