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Table 4 Summary of physiological properties and composition of B. napus cotyledons expressing AtGL3

From: The biochemical composition and transcriptome of cotyledons from Brassica napus lines expressing the AtGL3 transcription factor and exhibiting reduced flea beetle feeding

A. Insect behaviour and cotyledon biochemistry relative to Westar
Genotype FB Feeding Cotyledon Morphology Cotyledon Orientation Anthocyanin Accumulation Cell Wall Carbohydrates Cell Wall Lignin
AtGL3+a Decreased Abnormal Vertical Increased No Difference No Difference
K-5-8b Decreased Normal Vertical Decreased No Difference Decreased
B. Glucosinolate composition of transgenic lines relative to Westar
Genotype Total GS Progoitrinc Glucoraphaninc Gluconapinc 4-hydroxy-glucobrassicinc Glucobrassicinc 4-methoxy-glucobrassicinc
AtGL3+ Decreased No Diference No Difference Increased No Difference No Difference Decreased
K-5-8 No Difference No Difference No Difference Increased Decreased No Difference Decreased
C. Glucosinolate composition of the transgenic lines relative to one another.
Genotype Total GS Progoitrinc Glucoraphaninc Gluconapinc 4-hydroxy-glucobrassicinc Glucobrassicinc 4-methoxy-glucobrassicinc
AtGL3+ Decreased Decreased No Difference Increased No Difference Increased Decreased
K-5-8 - - - - - - -
  1. aAtGL3+, B. napus cv. Westar expressing AtGL3 under direction of CaMV 35S promoter
  2. bK-5-8, B. napus cv. Westar expressing AtGL3 under direction of CaMV 35S promoter as well as a TTG1 RNAi construct
  3. cProgoitrin, 2-hydroxy-3-butenyl-GS; Glucoraphanin, 4-methylsulfinylbutyl-GS; Gluconapin, 3-butenyl-GS; 4-hydroxyglucobrassicin, 4-hydroxy-3-indolylmethyl-GS; Glucobrassicin, 3-indolylmethyl-GS; 4-methoxyglucobrassicin, 4-methoxy-3-indolylmethyl-GS