Fig. 5
From: Chilling-induced DNA Demethylation is associated with the cold tolerance of Hevea brasiliensis
Long-term cold treatment induced demethylation of promoters. Seedlings of H. brasiliensis were treated at 19 °C for 1 month (1-CT), transferred to 28 °C for recovery for 1 month, and then treated at 19 °C for 1 month (2-CT). After recovery for 1 month, the seedlings were again cold-treated for 1 month (3-CT). Leaf samples were collected after each treatment. Total DNA was isolated and treated with or without bisulphite. Methylation patterns were evaluated using CyMATE software. Probable TGN, THG, and THH demethylation sites were identified by the software and projected symbolically. The percent of each demethylation type were indicated at the right side. (a) A 324-bp region of the HICE1 promoter. (b) A 318-bp region of the HbCBF2 promoter. (c) A 287-bp fragment of the HbMET promoter