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Table 1 Quantification of in vivo and in vitro phenotypes conferred by chimeras

From: Dissection of MAPK signaling specificity through protein engineering in a developmental context

Name

Kinase Activity (normalized to MKK5DD)

SPCH stage inhibition of linage initiation

FAMA stage phenotypes (as percentage of total T1 seedlings)

KI-MPK3

KI-MPK6

n

%

n

Inhibited

WT

GC clusters

AVG

SE

AVG

SE

Small

Large

Total

YFP control

      

24

0.00

91.67

8.33

0.00

8.33

MKK5DD

34.485

9.052

38.259*

2.308

69

78.26

25

0.00

76.00

24.00

0.00

24.00

MKK7ED

6.836

4.808

10.867

5.726

93

100.00

50

20.00

26.00

0.00

54.00

54.00

N7-MKK5DD

5.500

1.894

21.448

9.176

75

26.67

125

8.80

32.00

48.80

10.40

59.20

N5-MKK7ED

15.889

7.052

35.235*

1.457

22

86.36

47

93.62

6.38

0.00

0.00

0.00

MKK5DD-C7

13.888

9.753

25.666*

2.530

47

87.23

44

65.91

29.55

4.55

0.00

4.55

MKK5DD-7A

45.980

22.674

26.287

4.947

114

12.28

98

0.00

97.96

2.04

0.00

2.04

MKK5DD-7B

46.746

22.671

38.718*

0.750

20

10.00

70

50.00

50.00

0.00

0.00

0.00

MKK7ED-C5

3.877

2.091

0.728

0.148

43

13.95

19

0.00

78.95

21.05

0.00

21.05

MKK7ED-CDR5

2.451

1.242

1.303*

0.312

89

98.81

30

96.67

3.33

0.00

0.00

0.00

MKK7ED-CPR5

15.844

11.444

5.904

2.422

105

0.00

36

2.78

91.67

5.56

0.00

5.56

MKK7ED-5A

15.440

12.606

5.219

4.017

125

88.00

54

81.48

14.81

3.70

0.00

3.70

MKK7ED-5B

7.238

4.545

1.317

0.200

198

16.67

60

0.00

48.33

40.00

11.67

51.67

N7-MKK5DD-C7

3.526

1.153

2.411

0.915

91

54.95

43

2.33

13.95

13.95

69.77

83.72

N5-MKK7ED-C5

4.724

2.876

1.439

0.673

51

72.55

46

0.00

71.74

28.26

0.00

28.26

MPK3, not shown

    

49

0.00

50

0.00

82.00

18.00

0.00

18.00

MPK3D193G/E197A

    

18

0.00

52

0.00

80.77

19.23

0.00

19.23

MPK3T119C, not shown

    

43

0.00

89

0.00

86.52

13.48

0.00

13.48

MPK6, not shown

    

122

0.00

45

0.00

84.44

15.56

0.00

15.56

MPK6D218G/E222A

    

6

100.00

27

0.00

77.78

22.22

0.00

22.22

MPK6Y144C, not shown

    

35

0.00

21

0.00

90.48

9.52

0.00

9.52

  1. Kinase activity is determined in triplicates with the exception of those marked with “*” that were done in duplicates. Averages (AVG) and Standard Errors (SE) are represented in the table. Phenotypic categories for FAMA stage phenotypes are described in Methods